Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-29T09:47:04.007Z Has data issue: false hasContentIssue false
  • English
  • Français

Crustacea in the Diet of Eledone Cirrhosa (Mollusca: Cephalopoda) determined by Serological Methods

Published online by Cambridge University Press:  11 May 2009

P. R. Boyle ,
M. S. Grisley  and
G. Robertson
P. R. Boyle
Affiliation:
Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen AB9 2TN
M. S. Grisley
Affiliation:
Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen AB9 2TN
G. Robertson
Affiliation:
Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen AB9 2TN
Get access Rights & Permissions [Opens in a new window]

Extract

Serological methods for prey identification have been applied to the gut contents of a field sample of 100 Eledone cirrhosa from the Moray Firth and 7 from the Sound of Jura. Protein extracts from the crop were electrophoresed (Laurell rockets) into antisera raised against potential crustacean prey species, Cancer pagurus, Carcinus maenas, Crangon crangon, Liocarcinus spp. and Nephrops norvegicus. The strengths of the resulting reactions were evaluated on the basis of peak height, staining density and the number of homocentric peaks of immune precipitates. Sixty-two of the 100 Moray Firth animals gave a positive reaction to one or more of the antisera and the most critical assessment of the results ranked the incidence of the prey as Liocarcinus (15) > Nephrops (10) > Cancer (8) > Crangon (2) > Carcinus (0), a total of 35 reactions from 28 animals. Absence of a reaction in the 38 animals from which appreciable sample volumes were also obtained indicates that alternative prey had been consumed. Of the 7 Sound of Jura animals, 4 gave a positive reaction to Nephrops alone. A total of only 24 animals gave any indication of diet by visual recognition of remains. The value and difficulties of the methodology are discussed.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 66 , Issue 4 , November 1986 , pp. 867 - 879
Copyright
Copyright © Marine Biological Association of the United Kingdom 1986

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ambrose, R. F., 1983. Midden formation by octopuses: the role of biotic and abiotic factors. Marine Behaviour and Physiology, 10, 137–144.CrossRefGoogle Scholar
Ambrose, R. F., 1984. Food preferences, prey availability, and the diet of Octopus bimaculatus Verrill. Journal of Experimental Marine Biology and Ecology, 77, 29–44.Google Scholar
Ambrose, R. F. & Nelson, B. V., 1983. Predation by Octopus vulgaris in the Mediterranean. Marine Ecology, 4, 251–261.CrossRefGoogle Scholar
Boyle, P. R., 1986. A descriptive ecology of Eledone cirrhosa (Mollusca: Cephalopoda) in Scottish waters. Journal of the Marine Biological Association of the United Kingdom, 66, 855–865.CrossRefGoogle Scholar
Clarke, H. G. M. & Freeman, T., 1966. A quantitative immunoelectrophoresis method (Laurell electrophoresis). Protides of the Biological Fluids, 14, 503–509.Google Scholar
Feller, R. J. & Gallagher, E. D., 1982. Antigenic similarities among estuarine soft-bottom benthic taxa. Oecologia, 52, 305–310.Google Scholar
Feller, R. J., Taghon, G. L., Gallagher, E. D., Kenny, G. E. & Jumars, P. A., 1979. Immunological methods of food web analysis in a soft-bottom benthic community. Marine Biology, 54, 61–74.Google Scholar
Grisley, M. S. & Boyle, P. R. 1985. A new application of serological techniques to gut content analysis. Journal of Experimental Marine Biology and Ecology, 90, 1–9.CrossRefGoogle Scholar
Hartwick, B., Tulloch, L. & Macdonald, S., 1981. Feeding and growth of Octopus dofleini (Wülker). Veliger, 24, 129–138.Google Scholar
Hirtle, R. W. M., 1978. Observations on feeding and grooming behaviour of captive squid, Illex illecebrosus (Lesueur, 1821). Technical Report. Fisheries and Marine Service, Canada, no. 833, 13.1–13.5.Google Scholar
Hurley, A. C, 1976. Feeding behavior, food consumption, growth and respiration of the squid Loligo opalescens raised in the laboratory. Fishery Bulletin. National Oceanic and Atmospheric Administration of the United States, 74, 176–182.Google Scholar
Laurell, C.-B., 1966. Quantitative estimation of proteins by electrophoresis in agarose gel, containing antibodies. Analytical Biochemistry, 15, 45–52.Google Scholar
Maldonado, H., 1963. The positive learning process in Octopus vulgaris. Zeitschrift fur vergleichende Physiologie, 47, 191–214.CrossRefGoogle Scholar
Martins, H. R., 1982. Biological studies of the exploited stock of Loligo forbesi (Mollusca: Cephalopoda) in the Azores. Journal of the Marine Biological Association of the United Kingdom, 62, 799–808.Google Scholar
Messenger, J. B., 1968. The visual attack of the cuttlefish Sepia officinalis. Animal Behaviour, 16, 342–357.Google Scholar
Moriyasu, M. 1981. Biologie des Pêches de Céphalopodes Benthiques. Application aux Eledones, Eledone cirrhosa (Lam., 1798) du Golfe du Lion. Thèse Doctorat, 3ème cycle, Université des Sciences et Techniques du Languedoc, France.Google Scholar
Moynihan, M. & Rodaniche, A. F., 1982. The Behaviour and Natural History of the Caribbean Reef Squid Sepioteuthis sepioidea. Berlin: Verlag Paul Parey. [Advances in Ethology, no. 25; Journal of Comparative Ethology, supplement 25.]Google Scholar
Nigmatullin, C. M. & Ostapenko, A. A. 1976. Feeding of Octopus vulgaris Lam. from the northwest African coast. International Council for the Exploration of the Sea (CM. Papers and Reports), K:6, 15 pp. [Mimeo.]Google Scholar
Nixon, M., 1984. Is there external digestion in Octopus? Journal of Zoology, 202, 441–447.Google Scholar
Nixon, M., 1986. Cephalopod diets. In CephalopodLife Cycles, vol. 2 (ed. Boyle, P. R.), pp. 201–219. London: Academic Press.Google Scholar
Sanchez, P., 1981. Régime alimentaire d 'Eledone cirrosa (Lamarck, 1798) (Mollusca: Cephalopoda) dans la Mer Catalane. Rapport et proces-verbaux des reunions. Commission internationale pour I'exploration scientifique de la Mer Méditerranée, 27(5), 209–212.Google Scholar
Vovk, A. N., 1983. Food Relations of Long-finned Squid, Loligo pealei Lesueur, in the Northwest Atlantic and its Position in the Ecosystem. North Atlantic Fisheries Organisation. [SCR Document, 83/IX/83.]Google Scholar
Yarnall, J. L., 1969. Aspects of the behaviour of Octopus cyanea Gray. Animal Behaviour, 17, 747–754.Google Scholar