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The Effect of Storage Temperature on the Enumeration of Epifluorescence-Detectable Bacterial Cells in Preserved Sea-Water Samples

Published online by Cambridge University Press:  11 May 2009

C. M. Turley  and
D. J. Hughes
C. M. Turley
Affiliation:
Plymouth Marine Laboratory, Citadel Hill, Plymouth, PL1 2PB
D. J. Hughes
Affiliation:
Plymouth Marine Laboratory, Citadel Hill, Plymouth, PL1 2PB
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Extract

Rates of decrease in epifluorescence-detectable bacteria in preserved sea-water samples changed significantly with temperature. This apparent cell loss was substantial even in preserved samples stored in a refrigerator, decreasing exponentially with time, with 56% apparent cell loss after 99 days’ storage. Storage at room temperature resulted in the greatest decrease in detectable cells, with 73% apparent cell loss after the same storage period. No significant difference was found between storage in two different containers. The apparent cell loss can be modelled in order to calculate the original cell numbers with confidence. However, models produced from one set of samples cannot be applied to the data from another.

Type
Short Communication
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 74 , Issue 1 , February 1994 , pp. 259 - 262
Copyright
Copyright © Marine Biological Association of the United Kingdom 1994

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