Hostname: page-component-745bb68f8f-5r2nc Total loading time: 0 Render date: 2025-01-16T05:59:05.605Z Has data issue: false hasContentIssue false
  • English
  • Français

Electrophoretic studies on blood proteins in an ecological series of isopod and amphipod species

Published online by Cambridge University Press:  11 May 2009

Wolfgang Wieser
Wolfgang Wieser
Affiliation:
Zoologisches Institut der Universität, Vienna, Austria
Get access Rights & Permissions [Opens in a new window]

Extract

An electrophoretic study of whole blood samples revealed the presence of haemocyanin and of various other proteins in the blood of fifteen species of marine and terrestrial amphipods and isopods.

The amount of haemocyanin per 1 mm3 of blood is independent of body weight over a range from 10 mg to 1 kg.

Under laboratory conditions and during starvation the haemocyanin level of the blood is lowered as compared with freshly caught specimens.

No relationship between moulting stage and haemocyanin content could be established in three oniscoid isopods, representing Ligia, Oniscus, and Porcellio.

In some species the densities of both haemocyanin and ‘slow proteins’ (i.e. proteins between starting line and haemocyanin) seem to vary in more or less the same direction, over at least part of the total range of variability. In the offshore Conilera, however, an indirect relationship seems to exist between these two protein fractions. This is reminiscent of the situation in Maia squinado described by Zuckerkandl (1960).

The slow protein fractions appear to play a number of roles in the species investigated. In Conilera they consist of various copper proteids (perhaps concerned with the synthesis of haemocyanin?), while in intertidal amphipods and some isopods they carry chromophilic groups that may be of importance in mechanisms of colour change. The colour of the blood of a given specimen of Orchestia gammarella and Marinogammarus marinus depends on the amount of the faster moving, blue chromoproteid present in the blood. These chromoproteids are considered to be carotenoproteids.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 45 , Issue 2 , June 1965 , pp. 507 - 523
Copyright
Copyright © Marine Biological Association of the United Kingdom 1965

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Allison, J. B. & COLE, W. H., 1940. The nitrogen, copper and hemocyanin content of the sera of several arthropods. J. biol. Chem., Vol. 135, pp. 259–65.Google Scholar
Berthet, J. & Berthet, P., 1963. Presence de l'hémocyanine dans l'hémolymphe des Isopodes et des Amphipodes. Archs int. Physiol. Biochim., T. 71. pp. 124–5.Google Scholar
Brown, N. A. & Hemingway, R. G., 1962. A rapid method for the determination of copper in biological tissues by means of zinc dibenzyldithiocarbamate. Res. Vet. Sci., Vol. 3, pp. 345–7.CrossRefGoogle Scholar
Charniaux-Legrand, H., 1951. Le cycle d'intermue des amphipodes et ses particularités chez les formes terrestres (Talitridae). Archs Zool. exp. gén., T. 88, pp. 178–204.Google Scholar
Drach, P., 1939. Mue et cycle d'intermue chez les Crustacés décapodes. Annls Inst. océanogr. Monaco, T. 19, pp. 103–391.Google Scholar
Frentz, R., 1960. Contribution a l'etude biochimique du milieu intérieur de Carcinus maenas Linné. 176 pp. Nancy: Soc. Impr. typographiques.Google Scholar
Goodwin, T. W., 1960. Biochemistry of pigments. In The Physiology of Crustacea (Waterman, T. H., ed.), Vol. 1, pp. 101–40. New York and London: Academic Press.Google Scholar
Kerkut, G. A., Moritz, P. M. & Munday, K. A., 1961. Variations of copper concentrations in Carcinus maenas. Cah. Biol. mar., T. 2, pp. 399–408.Google Scholar
Klotz, I. M., Schlesinger, A. H. & Tietze, F., 1948. Comparison of the binding ability of hemocyanin and serum albumin for organic ions. Biol. Bull. mar. biol. Lab., Woods Hole, Vol. 94, pp. 40–4.Google Scholar
Manwell, C. 1960. Comparative physiology: blood pigments. A. Rev. Physiol., Vol. 22, pp. 91–244.CrossRefGoogle ScholarPubMed
Manwell, C. & Baker, C. M., 1963. Starch gel electrophoresis of sera from some marine arthropods: studies on the heterogeneity of hemocyanin and on a ‘ceruloplasmin-like protein’. Comp. Biochem. Physiol., Vol. 8, pp. 193–208.Google Scholar
Przibram, H. & Lederer, E., 1933. Das Tiergrün der Heuschrecken als Mischung aus Farbstoffen. Anz. Akad. Wiss. Wien, math.-naturw. KL, Jg. 70, pp. 163–5.Google Scholar
ProsserLadd, C. (ed.), 1952. Comparative Animal Physiology. Philadelphia and London: W. B. Saunders Co.Google Scholar
Scholander, P. F., Flagg, W., Walters, V. & Irving, L., 1953. Climatic adaptation i n arctic and tropical poikilotherms. Physiol. Zool., Vol. 26, pp. 67–92.Google Scholar
Schulz, F. N., 1925. Die Körpersäfte (Crustaceen). In Handbuch d. vergl., Physiol. (Herausgegeben von H. Winterstein), Bd. 1, pp. 669–746. Jena: Gustav Fischer.Google Scholar
Smith, I. (ed.), 1960. Chromatographic and Electrophoretic Techniques. Vol. 2, Zone Electrophoresis. 215 pp. London: William Heinemann.Google Scholar
Stevenson, J. R., 1961. Polyphenol oxydase in the tegumental glands in relation to the molting cycle of the isopod crustacean Armadillidium vulgare. Biol. Bull. mar. biol. Lab., Woods Hole, Vol. 121, pp. 554–60.Google Scholar
Suneson, S., 1947, Colour change and chromatophore activators in Idothea. Acta Univ. lund., N.F. Adv. 2, Bd. 43, No. 5, 34 pp.Google Scholar
Tyler, A. & Metz, C. B., 1945. Natural heteroagglutinins in the serum of the spiny lobster, Panulirus interruptus. J. exp. Zool., Vol. 100, pp. 387–406.Google Scholar
Wieser, W., 1961. Copper in isopods. Nature, Lond., Vol. 191, p. 1020.Google Scholar
Wieser, W., 1962. Parameter des Sauerstoffverbrauches. I. Der Sauersoffverbrauch einiger Landisopoden. Z. vergl. Physiol., Bd. 45, pp. 247–71.Google Scholar
Wieser, W., 1965 a. Elektrophoretischer Nachweis von Hämocyanin und anderen Proteinen im Blut von Isopoden und Amphipoden. Die Naturwissenschaften (in the Press).Google Scholar
Wieser, W., 1965 b. Über die Häutung von Porcellio scaber Latr. Verh. dt. Zool. Ges. Kiel (in the Press).Google Scholar
Wieser, W. & Makart, H., 1961. Der Sauerstoffverbrauch und der Gehalt an Ca, Cu und einigen anderen Spurenelementen bei terrestrischen Asseln. Z. Naturf., Bd. 16b, pp. 816–19.Google Scholar
Zuckerkandl, E., 1960. Hémocyanine et cuivre chez un crustacé décapode, dans leurs rapports avec le cycle d'intermue Annls. Inst océanogr., Monaco, T. 38, pp. 1–122.Google Scholar