Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-13T04:03:45.220Z Has data issue: false hasContentIssue false

Morphology and molecular analysis of the 18S rRNA gene of oyster shell borers, Polydora species (Polychaeta: Spionidae), from Japan and Australia

Published online by Cambridge University Press:  23 January 2013

Waka Sato-Okoshi*
Affiliation:
Laboratory of Biological Oceanography, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan
Hirokazu Abe
Affiliation:
Laboratory of Biological Oceanography, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan
*
Correspondence should be addressed to: W. Sato-Okoshi, Laboratory of Biological Oceanography, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan email: wsokoshi@bios.tohoku.ac.jp

Abstract

Nuclear 18S rRNA gene sequences of oyster shell borers, Polydora websteri, P. calcarea and P. haswelli (Polychaeta: Spionidae), were determined for the first time. A wide range of morphological variation, particularly with regard to pigmentation, is observed among these three species. This variation was characterized at the species level. Black pigmentation pattern on the palps is suggested to be a key characteristic for species determination. Polydora websteri and P. haswelli were sister species. This is the first record of P. calcarea from Japan and Australia and P. haswelli from Japan.

Type
Research Article
Copyright
Copyright © Marine Biological Association of the United Kingdom 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Benham, W.B. (1896) Archiannelida, Polychaeta, and Myzostomaria. In Harmer, S.F. and Shipley, A.E. (eds) The Cambridge natural history. London: Macmillan & Co. Ltd, pp. 241334.Google Scholar
Blake, J.A. (1996) Family Spionidae Grube, 1850. In Blake, J.A. , Hilbig, B. and Scott, P.H. (eds) Taxonomic atlas of the benthic fauna of the Santa Maria Basin and Western Santa Barbara Channel. Volume 6. The Annelida. Part 3. Polychaeta: Orbiniidae to Cossuridae. Santa Barbara, CA: Santa Barbara Museum of Natural History, pp. 81224.Google Scholar
Blake, J. A. and Kudenov, J. D. (1978) The Spionidae (Polychaeta) from Southeastern Australia and adjacent areas with a revision of the genera. Memoirs of the National Museum of Victoria 39, 171280.CrossRefGoogle Scholar
Cohen, A.N. and Carlton, J.T. (1998) Accelerating invasion rate in a highly invaded estuary. Science 279, 555558.CrossRefGoogle Scholar
Handley, S.J. and Bergquist, P.R. (1997) Spionid polychaete infestations of intertidal Pacific oysters Crassostrea gigas (Thunberg), Mahurangi Harbor, northern New Zealand. Aquaculture 153, 191205.CrossRefGoogle Scholar
Lleonart, M., Handlinger, J. and Powell, M. (2003) Spionid mudworm infestation of farmed abalone (Haliotis spp.). Aquaculture 221, 8596.CrossRefGoogle Scholar
Manchenko, G.P. and Radashevsky, V.I. (1993) Genetic differences between two sibling species of the Polydora ciliata complex (Polychaeta: Spionidae). Biochemical Systematics and Ecology 21, 543548.CrossRefGoogle Scholar
Manchenko, G.P. and Radashevsky, V.I. (1998) Genetic evidence for two sibling species within Polydora cf. ciliata (Polychaeta: Spionidae) from the Sea of Japan. Marine Biology 131, 489495.CrossRefGoogle Scholar
Mori, K., Sato, W., Nomura, T. and Imajima, M. (1985) Infestation of the Japanese scallop Patinopecten yessoensis by the boring polychaetes, Polydora, on the Okhotsk Sea coast of Hokkaido, especially in Abashiri Waters. Bulletin of the Japanese Society of Scientific Fisheries 51, 371380. [In Japanese with English abstract.]CrossRefGoogle Scholar
Mustaquim, J. (1988) Electrophoretic variation of isozymes in Polydora ciliata complex (Polychaeta: Spionidae). Comparative Biochemistry and Physiology—Part B: Biochemistry and Molecular Biology 91, 197205.CrossRefGoogle Scholar
Nishitani, G., Nagai, S., Hayakawa, S., Kosaka, Y., Sakurada, K., Kamiyama, T. and Gojobori, T. (2012) Mutiple plastids collected by the dinoflagellate Dinophysis mitra through Kleptoplastidy. Applied and Environmental Microbiology 78, 813821.CrossRefGoogle Scholar
Radashevsky, V.I. (1999) Description of the proposed lectotype for Polydora websteri Hartman in Loosanoff & Engle, 1943 (Polychaeta: Spionidae). Ophelia 51, 107113.CrossRefGoogle Scholar
Radashevsky, V.I. and Olivares, C. (2005) Polydora uncinata (Polychaeta: Spionidae) in Chile: an accidental transportation across the Pacific. Biological Invasions 7, 489496.CrossRefGoogle Scholar
Radashevsky, V.I. and Pankova, V.V. (2006) The morphology of two sibling sympatric Polydora species (Polydora: Spionidae) from the Sea of Japan. Journal of the Marine Biological Association of the United Kingdom 86, 245252.CrossRefGoogle Scholar
Radashevsky, V.I. and Williams, J.D. (1998) Polydora websteri Hartman in Loosanoff & Engle, 1943 (Annelida, Polychaeta): proposed conservation of the specific name by a ruling that it is not to be treated as a replacement for P. caeca Webster, 1879, and designation of a lectotype for P. websteri. Bulletin of Zoological Nomenclature 55, 212216.CrossRefGoogle Scholar
Read, G.B. (2010) Comparison and history of Polydora websteri and P. haswelli (Polychaeta: Spionidae) as mud-blister worms in New Zealand shellfish. New Zealand Journal of Marine and Freshwater Research 44, 118.CrossRefGoogle Scholar
Richlen, M.L. and Barber, P.H. (2005) A technique for the rapid extraction of microalgal DNA from single live and preserved cells. Molecular Ecology Notes 5, 688691.CrossRefGoogle Scholar
Rousset, V., Rouse, G.W., Siddal, M.E., Tillier, A. and Pleijel, F. (2004) The phylogenetic position of Siboglinidae (Annelida) inferred from 18S rRNA, 28S rRNA and morphological data. Cladistics 20, 518533.CrossRefGoogle ScholarPubMed
Sato-Okoshi, W. (1999) Polydorid species (Polychaeta: Spionidae) in Japan, with descriptions of morphology, ecology and burrow structure. 1. Boring species. Journal of the Marine Biological Association of the United Kingdom 79, 831848.CrossRefGoogle Scholar
Sato-Okoshi, W. (2000) Polydorid species (Polychaeta, Spionidae) in Japan, with descriptions of morphology, ecology and burrow structure. 2 Non-boring species. Journal of the Marine Biological Association of the United Kingdom 80, 443456.CrossRefGoogle Scholar
Sato-Okoshi, W., Okoshi, K. and Shaw, J. (2008) Polydorid species (Polychaeta: Spionidae) in south-western Australian waters with special reference to Polydora uncinata and Boccardia knoxi. Journal of the Marine Biological Association of the United Kingdom 88, 491501.CrossRefGoogle Scholar
Sato-Okoshi, W., Okoshi, K., Koh, B.-S., Kim, Y.-H. and Hong, J.-S. (2012) Polydorid species (Polychaeta: Spionidae) associated with commercially important mollusk shells in Korean waters. Aquaculture 350/353, 8290.CrossRefGoogle Scholar
Simon, C.A., Ludford, A. and Wynne, S. (2006) Spionid polychaetes infesting cultured abalone Haliotis midae in South Africa. African Journal of Marine Science 28, 167171.CrossRefGoogle Scholar
Struck, T.H. and Purschke, G. (2005) The sister group relationship of Aeolosomatida and Potamodrilidae (Annelida: ‘Polychaeta')—a molecular phylogenetic approach based on 18S rDNA and cytochrome oxidase I. Zoologischer Anzeiger 243, 281293.CrossRefGoogle Scholar
Tamura, K., Dudley, J., Nei, M. and Kumar, S. (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 15961599.CrossRefGoogle ScholarPubMed
Thompson, J.D., Higgins, D.G. and Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22, 46734680.CrossRefGoogle ScholarPubMed
Walker, L.M. (2011) A review of the current status of the Polydora-complex (Polychaeta: Spionidae) in Australia and a checklist of recorded species. Zootaxa 2751, 4062.CrossRefGoogle Scholar
Williams, J.D. and Radashevsky, V.I. (1999) Morphology, ecology, and reproduction of a new species from the east coast of North America. Ophelia 51, 115127.CrossRefGoogle Scholar