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Effects of salinity stress on cellular location of elements and photosynthesis in Ramalina canariensis Steiner

Published online by Cambridge University Press:  01 February 2011

Paula MATOS ,
João CARDOSO-VILHENA ,
Rui FIGUEIRA  and
A. Jorge SOUSA
Paula MATOS
Affiliation:
Universidade de Lisboa, Jardim Botânico, Museu Nacional de História Natural, R. Escola Politécnica 58, 1250-102 Lisboa, Portugal. Email: paulague@gmail.com. Universidade de Lisboa, Faculdade de Ciências (FCUL), Centro de Biologia Ambiental (CBA), Edifício C2, 5° Piso, Campo Grande 1749-016 Lisboa, Portugal.
João CARDOSO-VILHENA
Affiliation:
Universidade de Lisboa, Jardim Botânico, Museu Nacional de História Natural, R. Escola Politécnica 58, 1250-102 Lisboa, Portugal. Email: paulague@gmail.com. Universidade Atlântica, Antiga Fábrica da Pólvora de Barcarena, 2745-615 Barcarena, Portugal.
Rui FIGUEIRA
Affiliation:
Instituto de Investigação Científica Tropical, Jardim Botânico Tropical, Trav. Conde da Ribeira 9, 1300-142 Lisboa, Portugal. Instituto Superior Técnico, CERENA-Centro de Recursos Naturais e Ambiente, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
A. Jorge SOUSA
Affiliation:
Instituto Superior Técnico, CERENA-Centro de Recursos Naturais e Ambiente, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
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Abstract

The impact of incubation in saline solutions of different concentrations on the uptake and cellular location of essential elements (Na+, K+, Mg2+ and Ca2+), and its effects on membrane integrity and on the photosynthetic apparatus, were investigated in the lichen Ramalina canariensis Steiner. Saline incubation resulted in a rapid uptake of Na+ and Mg2+ in the cell wall fraction, whereas in the intracellular fraction the accumulation of Na+ was slower. No changes were observed for intracellular Mg2+, suggesting that no generalized membrane damage occurred. Concomitantly with the increase in intracellular Na+, there was a specific loss of K+ from the cell interior, indicating that membrane permeability may have been compromised. Incubation in a 100% artificial sea water solution reduced the maximum photochemical efficiency of Photosystem II (Fv/Fm) by 17% after 5 min, and this inhibition increased with incubation time. In samples incubated in 100% artificial sea water solution for 2 h followed by 2 h incubation in deionized water, ion distribution and Fv/Fm did not recover to control values. The present findings show the importance of determining the cellular location of elements when assessing their physiological impact. Results indicate that saline stress may irreversibly impair photosynthesis, thus compromising lichen vitality.

Keywords

cation exchangecellular fractionchlorophyll fluorescencelichenssaline stress
Type
Research Article
Information
The Lichenologist , Volume 43 , Issue 2 , March 2011 , pp. 155 - 164
Copyright
Copyright © British Lichen Society 2011

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