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Testing morphology-based delimitation of Vulpicida juniperinus and V. tubulosus (Parmeliaceae) using three molecular markers

Published online by Cambridge University Press:  08 October 2012

Kristiina MARK ,
Lauri SAAG ,
Andres SAAG ,
Arne THELL  and
Tiina RANDLANE
Kristiina MARK
Affiliation:
University of Tartu, Institute of Ecology and Earth Sciences, Department of Botany, Lai 38, 51005 Tartu, Estonia
Lauri SAAG*
Affiliation:
Estonian Biocentre, Riia 23b, 51010 Tartu, Estonia. Email: lauri.saag@ut.ee
Andres SAAG
Affiliation:
University of Tartu, Institute of Ecology and Earth Sciences, Department of Botany, Lai 38, 51005 Tartu, Estonia
Arne THELL
Affiliation:
The Biological Museums, Lund University, Lund, Sweden
Tiina RANDLANE
Affiliation:
University of Tartu, Institute of Ecology and Earth Sciences, Department of Botany, Lai 38, 51005 Tartu, Estonia
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Abstract

The delimitation of two morphologically similar and not easily separable Vulpicida species, V. juniperinus and V. tubulosus, is analyzed using nuclear ITS and Mcm7, and mitochondrial SSU DNA sequences. Seventy-nine Vulpicida specimens, most from the two focal taxa, are included in the three-locus gene tree. The results from Bayesian and parsimony analyses are presented. There are strong conflicts between the single locus gene trees. Vulpicida juniperinus and V. tubulosus are divided into two clearly distinguished groups in the ITS and concatenated B/MCMC tree. However, these species are mixed in both clades, appearing polyphyletic. Currently accepted V. juniperinus and V. tubulosus are not distinct according to the loci studied. Vulpicida pinastri appears monophyletic based on the available sequences.

Keywords

gene treeITSlichenized fungiMcm7molecular phylogeneticsMrBayesmtSSUtaxonomy
Type
Research Article
Information
The Lichenologist , Volume 44 , Issue 6 , November 2012 , pp. 757 - 772
Copyright
Copyright © British Lichen Society 2012

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