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Confocal laser scanning microscopy method for in vivo bryozoan larvae identification

Published online by Cambridge University Press:  17 May 2010

A. Tsyganov-Bodounov  and
David O.F. Skibinski
A. Tsyganov-Bodounov*
Affiliation:
School of the Environment & Society, Biological Sciences, University of Wales, Swansea, SA2 8PP, UK
David O.F. Skibinski
Affiliation:
Institute of Life Sciences, School of Medicine, University of Wales, Swansea, SA2 8PP, UK
*
Correspondence should be addressed to: A. Tsyganov-Bodounov, School of the Environment & Society, Biological Sciences, University of Wales, Swansea, SA2 8PP, UK emails: a.tsyganov@liverpool.ac.uk, atsyganov@gmail.com
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Abstract

Knowledge of bryozoan larval types is limited to a relatively small number of species. Further information is needed to establish traits of evolutionary significance and thus advance bryozoan taxonomy. Morphological classification of bryozoan larvae has previously focused on electron microscopy, epi-fluorescence microscopy and lightfield microscopy methods. These are either relatively time consuming and require fixed material, or they fail to reveal surface features of larvae. A method is presented here, based on confocal laser scanning microscopy, which allows in vivo 3-D imaging of larvae. The method could also be used for imaging other marine invertebrate larvae of comparable size.

Keywords

Bryozoalarvaeconfocal laser scanning microscopymorphology
Type
Research Article
Information
Marine Biodiversity Records , Volume 3 , June 2010 , e54
Copyright
Copyright © Marine Biological Association of the United Kingdom 2010

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