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Individual Cell-Based Model for In-Vitro Mesothelial Invasionof Ovarian Cancer

Published online by Cambridge University Press:  03 February 2010

C. Giverso ,
M. Scianna ,
L. Preziosi ,
N. Lo Buono  and
A. Funaro
C. Giverso
Affiliation:
Department of Mathematics, Politecnico di Torino, Corso Duca degli Abruzzi, 24 10129 Torino, Italy
M. Scianna
Affiliation:
Department of Mathematics, Politecnico di Torino, Corso Duca degli Abruzzi, 24 10129 Torino, Italy
L. Preziosi*
Affiliation:
Department of Mathematics, Politecnico di Torino, Corso Duca degli Abruzzi, 24 10129 Torino, Italy
N. Lo Buono
Affiliation:
Laboratory of Immunogenetics, Department of Genetics, Biology and Biochemistry, University of Turin Medical School, Via Santena 19, 10126 Torino, Italy
A. Funaro
Affiliation:
Laboratory of Immunogenetics, Department of Genetics, Biology and Biochemistry, University of Turin Medical School, Via Santena 19, 10126 Torino, Italy
*
* Corresponding author. E-mail:luigi.preziosi@polito.it
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Abstract

In vitro transmesothelial migration assays of ovarian cancer cells, isolated oraggregated in multicellular spheroids, are reproduced deducing suitable Cellular PottsModels (CPM). We show that the simulations are in good agreement with the experimentalevidence and that the overall process is regulated by the activity of matrixmetalloproteinases (MMPs) and by the interplay of the adhesive properties of the cellswith the extracellular matrix and between cells, both of the same type and of differenttypes. In particular, the process depends on the ability of the cell to induce theloosening of cadherin-mediated junctions. Coherently with experiments, it is found thatsingle cell invasion is more conservative with a crucial role played by MMPs. A similarimportant role is played in cell spheroid invasion, which in comparison is moredisruptive. It achieves monofocal or multifocal characteristics according to the relativeadhesion affinity among cells or between them and the mesothelial layer.

Keywords

ovarian cancer metastasismesotheliumcellular potts modelhybrid model
Type
Research Article
Information
Mathematical Modelling of Natural Phenomena , Volume 5 , Issue 1: Cell migration , 2010 , pp. 203 - 223
Copyright
© EDP Sciences, 2010

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