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Applications of Correlative Microscopy in Diagnostic and Investigative Pathology

Published online by Cambridge University Press:  02 July 2020

D. N. Howell  and
S. E. Miller
D. N. Howell
Affiliation:
Departments of Pathology, Duke University Medical Center, Durham, NC27710 and V. A. Medical Center, Durham, NC27705
S. E. Miller
Affiliation:
Departments of Microbiology and Pathology, Duke University Medical Center, Durham, NC27710
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Extract

Correlative microscopy is employed in a great variety of settings by both diagnostic and investigative pathologists. Combinations of conventional light microscopy (LM), immunohistology, and electron microscopy (EM) are used in a wide range of diagnostic settings, including the analysis of tumors, autoimmune disorders, and infectious diseases. Valuable diagnostic information is also frequently obtained by simultaneous or sequential examination of exfoliated or aspirated cell suspensions (cytopathology) and tissue sections (histopathology) from the same lesion. An even wider range of correlative microscopic methods is employed by pathologists in basic and clinical research. The rationales for using correlative techniques are many and varied, but in most cases fall within a limited number of categories.

Pathologists frequently use a second microscopic or preparative technique to improve on the resolution afforded by an initial technique. Electron microscopy is often used to refine the analysis of features initially detected by routine LM or immunohistology.

Type
Application of Classical and Novel Microscopy to Tissue Injury and Infectious Disease Pathogenesis
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1044 - 1045
Copyright
Copyright © Microscopy Society of America

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References

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