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Applications of Soft X-Ray and other Microscopy Techniques to Elucidate the Structure of Parasitic Metazoa

Published online by Cambridge University Press:  02 July 2020

W. J. Kozek ,
J. Brown ,
W. Meyer-Ilse ,
C. Larabell  and
M. Moronne
W. J. Kozek
Affiliation:
Department of Microbiology and Medical Zoology, Medical Sciences Campus, University of Puerto Rico, San JuanPR
J. Brown
Affiliation:
Center for X-Ray Optics, Advanced Light Source, E. O. Lawrence Berkeley National Laboratory, Berkeley, CA94720
W. Meyer-Ilse
Affiliation:
Center for X-Ray Optics, Advanced Light Source, E. O. Lawrence Berkeley National Laboratory, Berkeley, CA94720
C. Larabell
Affiliation:
Structural Biology Laboratory, Division of Life Sciences, E. O. Lawrence Berkeley National Laboratory, Berkeley, CA94720
M. Moronne
Affiliation:
Structural Biology Laboratory, Division of Life Sciences, E. O. Lawrence Berkeley National Laboratory, Berkeley, CA94720
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Extract

The small size of many parasitic organisms requires the use of election microscopy for adequate elucidation of their structure. While both transmission and scanning electron microscopy can provide complementary results which allow considerable degree of structural correlation, each technique has its inherent limitations. Since previous studies have demonstrated that soft X-ray microscopy could be used to study parasitic protozoa and provide new information, the objective of this study was to determine whether soft X-ray microscopy could also be used to elucidate the morphology of small metazoa to complement the data obtained by other microscopy techniques.

Newborn larvae, approximately 7 μm × 110 μm in size, of parasitic nematode Trichinella spiralis were used as a model system. Some of the larvae, deposited by adult females maintained in vitro, were isolated and processed for examination by transmission and scanning electron microscopy as described in our previous studies, others were fixed in 4% glutaraldehyde (Millonig's buffer) and examined in the X-ray microscope XM-1, and in the BioRad MRC 1024 confocal laser (krypton/argon) microscope of the Advanced Light Source, Berkeley National Laboratory.

Type
Biological Ultrastructure/Microbiology
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1156 - 1157
Copyright
Copyright © Microscopy Society of America

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References

1.Magowan, C. et al. Blood 86(1995)319CrossRefGoogle Scholar
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4. This research was supported by the Director, Office of Energy Research, Office of Basic Energy Sciences, Material Sciences Division of the U.S. Department of Energy, under contract No. DEAC03- 76SF00098, and by the ‘Research Centers in Minority Institutions’ Award RR-03051, from the National Center for Research Resources, National Institutes of Health for Research Resources, National Institutes of Health.Google Scholar