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Construction and Establishment of a New Environmental Chamber to Study Real-Time Cardiac Development

Published online by Cambridge University Press:  09 May 2007

Gülay Orhan ,
Stephan Baron ,
Kambiz Norozi ,
Jörg Männer ,
Oliver Hornung ,
Holger Blume ,
Judith Misske ,
Bodo Heimann ,
Armin Wessel  and
T. Mesud Yelbuz
Gülay Orhan
Affiliation:
Department of Pediatric Cardiology and Intensive Care Medicine, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany
Stephan Baron
Affiliation:
Hannover Center of Mechatronics, Leipniz University of Hannover, Appelstraße 11, 30167 Hannover, Germany
Kambiz Norozi
Affiliation:
Department of Pediatric Cardiology and Intensive Care Medicine, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany
Jörg Männer
Affiliation:
Department of Anatomy & Embryology, Georg-August-University of Göttingen, Kreuzbergring 36, 37075 Göttingen, Germany
Oliver Hornung
Affiliation:
Hannover Center of Mechatronics, Leipniz University of Hannover, Appelstraße 11, 30167 Hannover, Germany
Holger Blume
Affiliation:
Hannover Center of Mechatronics, Leipniz University of Hannover, Appelstraße 11, 30167 Hannover, Germany
Judith Misske
Affiliation:
Department of Pediatric Cardiology and Intensive Care Medicine, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany
Bodo Heimann
Affiliation:
Hannover Center of Mechatronics, Leipniz University of Hannover, Appelstraße 11, 30167 Hannover, Germany
Armin Wessel
Affiliation:
Department of Pediatric Cardiology and Intensive Care Medicine, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany
T. Mesud Yelbuz
Affiliation:
Department of Pediatric Cardiology and Intensive Care Medicine, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany
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Abstract

Heart development, especially the critical phase of cardiac looping, is a complex and intricate process that has not yet been visualized “live” over long periods of time. We have constructed and established a new environmental incubator chamber that provides stable conditions for embryonic development with regard to temperature, humidity, and oxygen levels. We have integrated a video microscope in the chamber to visualize the developing heart in real time and present the first “live” recordings of a chick embryo in shell-less culture acquired over a period of 2 days. The time-lapse images we show depict a significant time window that covers the most critical and typical morphogenetic events during normal cardiac looping. Our system is of interest to researchers in the field of embryogenesis, as it can be adapted to a variety of animal models for organogenesis studies including heart and limb development.

Keywords

heart developmentimagingcardiac looping
Type
BIOLOGICAL APPLICATIONS
Information
Microscopy and Microanalysis , Volume 13 , Issue 3 , June 2007 , pp. 204 - 210
Copyright
© 2007 Microscopy Society of America

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