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Detection of Giardia Trophozoites in Archival Pathology Specimens of Human Small Intestine

Published online by Cambridge University Press:  28 July 2005

P. Timothy Macechko ,
Harry van Keulen ,
Edward L. Jarroll ,
Thomas Mulgrew ,
Andrew Gurien  and
Stanley L. Erlandsen
P. Timothy Macechko
Affiliation:
Department of Cell Biology and Neuroanatomy, University of Minnesota Medical School, Minneapolis, MN 55455
Harry van Keulen
Affiliation:
Department of Biological, Geological and Environmental Sciences, Cleveland State University, 2399 Euclid Avenue, Cleveland, OH 44115
Edward L. Jarroll
Affiliation:
Department of Biology, Northeastern University, 360 Huntington Avenue, Boston, MA 02115
Thomas Mulgrew
Affiliation:
Department of Cell Biology and Neuroanatomy, University of Minnesota Medical School, Minneapolis, MN 55455
Andrew Gurien
Affiliation:
Department of Cell Biology and Neuroanatomy, University of Minnesota Medical School, Minneapolis, MN 55455
Stanley L. Erlandsen
Affiliation:
Department of Cell Biology and Neuroanatomy, University of Minnesota Medical School, Minneapolis, MN 55455
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Abstract

The examination of archival pathology specimens of human small intestine by light microscopy, field emission scanning electron microscopy (FESEM), and confocal scanning laser microscopy using fluorescent in situ hybridization (FISH) techniques was undertaken to better understand the epidemiology of Giardia. Giardia trophozoites were tentatively identified in the light microscope after hematoxylin and eosin (H&E) staining. The organisms were adherent to the intestinal epithelium where they were also associated with strands of mucus within the lumen. Fluorochrome-conjugated antisense oligonucleotide probes, developed for the 16S rRNA of Giardia lamblia and Giardia muris, were used in FISH experiments with confocal scanning laser microscopy. Positive identification of trophozoites could be obtained with the G. lamblia probe, but not with the G. muris probe. FESEM examination of serial sections adjacent to FISH-stained sections revealed trophozoites characterized by their morphological features. The 16S rDNA probes specifically distinguished different species of Giardia, but whether multiple infections can occur within an individual host could not be determined.

Keywords

Giardiafield emission scanning electron microscopy (FESEM)confocal scanning laser microscopyin situ hybridization (ISH)fluorescence in situ hybridization (FISH)rDNA probes
Type
Research Article
Information
Microscopy and Microanalysis , Volume 4 , Issue 4 , August 1998 , pp. 397 - 403
Copyright
© 2005 Microscopy Society of America

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