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Differential Uptake and Selective Permeability of Fusarochromanone (FC101), a Novel Membrane Permeable Anticancer Naturally Fluorescent Compound in Tumor and Normal Cells

Published online by Cambridge University Press:  16 September 2009

Brian D. Furmanski ,
Didier Dréau ,
Roy E. Wuthier  and
John W. Fuseler
Brian D. Furmanski
Affiliation:
Department of Chemistry and Biochemistry, University of South Carolina, 337 Graduate Science Research Center, Columbia, SC 29208
Didier Dréau
Affiliation:
Department of Biology, University of North Carolina-Charlotte, 9201 University City Blvd., Charlotte, NC 28223
Roy E. Wuthier
Affiliation:
Department of Chemistry and Biochemistry, University of South Carolina, 337 Graduate Science Research Center, Columbia, SC 29208
John W. Fuseler*
Affiliation:
Department of Cell Biology and Anatomy, University of South Carolina, School of Medicine Campus 661, B-58, Columbia, SC 29209
*
Corresponding author. E-mail: John.Fuseler@uscmed.sc.edu
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Abstract

The differential accumulation of fluorescent molecules in tumorigenic versus normal cells is a well-reported phenomenon and is the basis for photodiagnostic therapy. Through the use of confocal microscopy, the kinetic uptake and accumulation of fusarochromanone (FC101) was determined in two lines of living tumorigenic cells of mesenchymal-epithelial origin and normal fibroblast cells. Like other fluorescent cationic molecules, FC101 showed increased accumulation in tumorigenic cells; however, unlike other molecules, it appeared to be accumulated in a time-dependent manner. Also, unlike traditional fluorescent cationic molecules, FC101, a potent inhibitor of cell growth, showed preferential inhibition of tumorigenic B-16 melanoma cells and MCF7 cells derived from breast cancer adenocarcinoma when compared to normal cardiac fibroblasts. Further analysis of FC101's physicochemical properties using both experimentally obtained and simulated values revealed the likelihood of membrane permeation and oral bioavailability of the compound. These physicochemical properties of FC101 were also used to predict its intracellular localization lending credence to data observed by confocal microscopy.

Keywords

fusarochromanone (FC101)confocal microscopyimage analysistransformed cellsnormal cellsdrug uptake
Type
Biological Science Applications
Information
Microscopy and Microanalysis , Volume 15 , Issue 6 , December 2009 , pp. 545 - 557
Copyright
Copyright © Microscopy Society of America 2009

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