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Dilution and Magnification Effects on Image Analysis Applications in Activated Sludge Characterization

Published online by Cambridge University Press:  31 August 2010

D.P. Mesquita ,
O. Dias ,
R.A.V. Elias ,
A.L. Amaral  and
E.C. Ferreira
D.P. Mesquita
Affiliation:
IBB—Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal
O. Dias
Affiliation:
IBB—Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal
R.A.V. Elias
Affiliation:
Escola Superior de Tecnologia e de Gestão, Instituto Politécnico de Bragança, Campus de Santa Apolónia, Apartado 134, 5301-857 Bragança, Portugal
A.L. Amaral
Affiliation:
IBB—Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal Instituto Superior de Engenharia de Coimbra, Instituto Politécnico de Coimbra, Rua Pedro Nunes, Quinta da Nora, 3030-199 Coimbra, Portugal
E.C. Ferreira*
Affiliation:
IBB—Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal
*
Corresponding author. E-mail: ecferreira@deb.uminho.pt
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Abstract

The properties of activated sludge systems can be characterized using image analysis procedures. When these systems operate with high biomass content, accurate sludge characterization requires samples to be diluted. Selection of the best image acquisition magnification is directly related to the amount of biomass screened. The aim of the present study was to survey the effects of dilution and magnification on the assessment of aggregated and filamentous bacterial content and structure using image analysis procedures. Assessments of biomass content and structure were affected by dilutions. Therefore, the correct operating dilution requires careful consideration. Moreover, the acquisition methodology comprising a 100× magnification allowed data on aggregated and filamentous biomass to be determined and smaller aggregates to be identified and characterized, without affecting the accuracy of lower magnifications regarding biomass representativeness.

Keywords

activated sludgeimage analysisdilutionmagnificationaggregatesfilaments
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 16 , Issue 5 , October 2010 , pp. 561 - 568
Copyright
Copyright © Microscopy Society of America 2010

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References

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