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Evaluation and Microanalysis of Parasitic and Bacterial Agents of Egyptian Fresh Sushi, Salmo salar

Part of: Micrographia Collection

Published online by Cambridge University Press:  13 November 2019

Sara S. Abdel-Hakeem Open the ORCID record for Sara S. Abdel-Hakeem [Opens in a new window] ,
Ghada Abd-Elmonsef Mahmoud Open the ORCID record for Ghada Abd-Elmonsef Mahmoud [Opens in a new window]  and
Hanan H. Abdel-Hafeez Open the ORCID record for Hanan H. Abdel-Hafeez [Opens in a new window]
Sara S. Abdel-Hakeem
Affiliation:
Parasitology Lab, Zoology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt
Ghada Abd-Elmonsef Mahmoud
Affiliation:
Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut 71516, Egypt
Hanan H. Abdel-Hafeez*
Affiliation:
Department of Anatomy, Embryology and Histology, Faculty of Veterinary Medicine, Assiut University, Assiut 71516, Egypt (Previous PhD student) Department of Veterinary Sciences, Institute for Anatomy, Histology and Embryology, Ludwig Maximilian University, Veterinärstrasse 13, DE-80539 Munich, Germany
*
*Author for correspondence: Hanan H. Abdel-Hafeez, E-mail: hhmmzz91@gmail.com
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Abstract

The present study aimed to evaluate the quality of fresh sushi in Egypt. Fifty samples of sushi (Salmo salar) were collected from restaurants in Alexandria, Egypt. Paraffin, semi-thin and ultra-thin sections were used for parasitological analysis by light and transmission electron microscopy. Bacteria were isolated by the dilution plate and direct plate methods and identified by a Vitek system. Twenty (40%) of the total examined samples showed microsporidia and helminth metacercariae infections. Histochemical stains showed distinct pinkish-red pyriform microspores embedded in muscular tissue stained with Gram, periodic acid-Schiff (PAS), and Ziehl–Neelsen (ZN) stains. Semi-thin sections showed double membrane xenoma-inducing granulomas containing spores at different developmental stages. Empty sporophorous vesicles and free spores were observed in the electron microscopic images. A bacteriological assay showed forty samples (80%) contaminated with human pathogenic bacteria with the average total bacterial counts ranging from 32 to 526 CFU/g. Four species of human pathogenic bacteria were identified in the examined samples, namely Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, and Serratia plymuthica in 40, 38, 11, and 6 samples, respectively. These constitute the first record of fresh sushi product in Egypt and indicate the potential pathogenicity associated with raw seafood products.

Keywords

metacercariamicrosporepathogenic bacteriasushi
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 25 , Issue 6 , December 2019 , pp. 1498 - 1508
Copyright
Copyright © Microscopy Society of America 2019 

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