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Fine Structure of Neurally Differentiated iPS Cells Generated from a Multiple Sclerosis (MS) Patient: A Case Study

Published online by Cambridge University Press:  22 October 2014

Daniella Herszfeld ,
Natalie L. Payne ,
Aude Sylvain ,
Guizhi Sun ,
Claude C. Bernard ,
Joan Clark  and
Henry Sathananthan
Daniella Herszfeld
Affiliation:
Australian Regenerative Medicine Institute, Monash University, Clayton, VIC 3800, Australia
Natalie L. Payne
Affiliation:
Australian Regenerative Medicine Institute, Monash University, Clayton, VIC 3800, Australia
Aude Sylvain
Affiliation:
Australian Regenerative Medicine Institute, Monash University, Clayton, VIC 3800, Australia
Guizhi Sun
Affiliation:
Australian Regenerative Medicine Institute, Monash University, Clayton, VIC 3800, Australia
Claude C. Bernard
Affiliation:
Australian Regenerative Medicine Institute, Monash University, Clayton, VIC 3800, Australia
Joan Clark
Affiliation:
Monash Micro Imaging, Monash University, Clayton, VIC 3800, Australia
Henry Sathananthan*
Affiliation:
Australian Regenerative Medicine Institute, Monash University, Clayton, VIC 3800, Australia Department of Anatomy & Developmental Biology, Monash University, Clayton, VIC 3800, Australia
*
*Corresponding author. henry.sathananthan@monash.edu
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Abstract

We compared the characteristics of neural cells derived from induced pluripotent stem (iPS) cells from a patient with multiple sclerosis versus neurally differentiated control iPS cells of a healthy individual. The iPS cells were differentiated toward the oligodendrocyte lineage using a four-step protocol established for the differentiation of embryonic stem cells. The resulting cell population was immunostained on day 112 of differentiation for the presence of oligodendrocytes and analyzed by transmission electron microscopy (TEM). Both patient and control samples resembled a mixed population of neural cells rather than oligodendroglia of high purity, including neural stem cell-like cells and possibly oligodendrocytes demonstrable by TEM.

Keywords

multiple sclerosisneural differentiationinduced pluripotent stem cellsneural stem cellsoligodendrocytefluorescence microscopytransmission electron microscopy
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 20 , Issue 6 , December 2014 , pp. 1869 - 1875
Copyright
© Microscopy Society of America 2014 

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