Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-28T04:53:55.307Z Has data issue: false hasContentIssue false
  • English
  • Français

Fine Structure of Neurally Differentiated iPS Cells Generated from a Multiple Sclerosis (MS) Patient: A Case Study

Published online by Cambridge University Press:  22 October 2014

Daniella Herszfeld ,
Natalie L. Payne ,
Aude Sylvain ,
Guizhi Sun ,
Claude C. Bernard ,
Joan Clark  and
Henry Sathananthan
Daniella Herszfeld
Affiliation:
Australian Regenerative Medicine Institute, Monash University, Clayton, VIC 3800, Australia
Natalie L. Payne
Affiliation:
Australian Regenerative Medicine Institute, Monash University, Clayton, VIC 3800, Australia
Aude Sylvain
Affiliation:
Australian Regenerative Medicine Institute, Monash University, Clayton, VIC 3800, Australia
Guizhi Sun
Affiliation:
Australian Regenerative Medicine Institute, Monash University, Clayton, VIC 3800, Australia
Claude C. Bernard
Affiliation:
Australian Regenerative Medicine Institute, Monash University, Clayton, VIC 3800, Australia
Joan Clark
Affiliation:
Monash Micro Imaging, Monash University, Clayton, VIC 3800, Australia
Henry Sathananthan*
Affiliation:
Australian Regenerative Medicine Institute, Monash University, Clayton, VIC 3800, Australia Department of Anatomy & Developmental Biology, Monash University, Clayton, VIC 3800, Australia
*
*Corresponding author. henry.sathananthan@monash.edu
Get access

Abstract

We compared the characteristics of neural cells derived from induced pluripotent stem (iPS) cells from a patient with multiple sclerosis versus neurally differentiated control iPS cells of a healthy individual. The iPS cells were differentiated toward the oligodendrocyte lineage using a four-step protocol established for the differentiation of embryonic stem cells. The resulting cell population was immunostained on day 112 of differentiation for the presence of oligodendrocytes and analyzed by transmission electron microscopy (TEM). Both patient and control samples resembled a mixed population of neural cells rather than oligodendroglia of high purity, including neural stem cell-like cells and possibly oligodendrocytes demonstrable by TEM.

Keywords

multiple sclerosisneural differentiationinduced pluripotent stem cellsneural stem cellsoligodendrocytefluorescence microscopytransmission electron microscopy
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 20 , Issue 6 , December 2014 , pp. 1869 - 1875
Copyright
© Microscopy Society of America 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Burkitt, H.G., Young, B. & Heath, J.W. (1993). Wheater’s Functional Histology: A Text and Colour Atlas, 3rd ed. Edinburgh, UK: Churchill Livingstone, 407pp.Google Scholar
Hu, B.Y., Du, Z.W. & Zhang, S.C. (2009). Differentiation of human oligodendrocytes from pluripotent stem cells. Nat Protoc 4, 1614–1622.Google Scholar
Sathananthan, A.H. (2011). Neural stem cells in neurospheres, embryoid bodies and CNS of human embryos. Microsc Microanal 17, 520–527.Google Scholar
Sathananthan, A.H., Selvaraj, K. & Clark, J. (2011). The fine structure of human germ layers in vivo: Clues to the early differentiation of embryonic stem cells (ESC) in vitro . Reprod BioMed Online 17, 227–233.CrossRefGoogle Scholar
Sathananthan, A.H., Selvaraj, K. & Clark, J. (2012). Fine structure of progenitor cells in early ectopic human embryos. Reprod BioMed Online 25, 315–321.Google Scholar
Sathananthan, A.H. & Trounson, A. (2007). In vitro differentiation of human embryonic stem cells. In Human Embryonic Stem Cells: A Practical Handbook, Sullivan, S., Cowan, C. & Eggan, K. (Eds.), pp. 149–168. NJ, USA: Wiley Press.Google Scholar
Sheng, H.Z., Martenson, R.E., Grgacic, E.V., Dowse, C.A., Carnegie, R.L. & Bernard, C.C.A. (1988). Electro immuno blotting of myelin basic protein peptides: a novel approach to rapid characterization of antigenic specificties of monoclonal and polyclonal anti-MBP antibodies. J Neuroimmunol 17, 183–192.Google Scholar
Song, B., Sun, G., Herszfeld, D., Sylvain, A., Campanale, N.V., Hirst, C.E., Caine, S., Parkington, H.C., Tonta, M.A., Coleman, H.A., Short, M., Ricardo, S.D., Reubinoff, B. & Bernard, C.C.A. (2012). Neural differentiation of patient specific iPS cells as a novel approach to study the pathophysiology of multiple sclerosis. Stem Cell Res 8, 259–273.Google Scholar
Wang, S., Bates, J., Li, X., Schanz, S., Chandler-Millitello, D., Levine, C., Maherali, N., Studer, L., Hochedlinger, K., Windrem, M. & Goldman, S.A. (2013). Human iPSC-derived oligodendrocyte progenitor cells can myelinate and rescue a mouse model of congenital hypomyelination. Cell Stem Cell 12, 252–264.Google Scholar
Yang, N., Zuchero, J.B., Ahlenius, H., Marro, S., Ng, Y.H., Vierbuchen, T., Hawkins, J.S., Geissler, R., Barres, B.A. & Wernig, M. (2013). Generation of oligodendroglial cells by direct lineage conversion. Nat Biotech 31, 434–439.CrossRefGoogle ScholarPubMed