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Large Area Cryo-Planing of Vitrified Samples Using Broad-Beam Ion Milling

Published online by Cambridge University Press:  12 October 2015

Irene Y. T. Chang  and
Derk Joester
Irene Y. T. Chang
Affiliation:
Department of Materials Science and Engineering, Northwestern University, 2220 Campus Drive, Evanston, IL 60208, USA
Derk Joester*
Affiliation:
Department of Materials Science and Engineering, Northwestern University, 2220 Campus Drive, Evanston, IL 60208, USA
*
*Corresponding author.d-joester@northwestern.edu
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Abstract

On account of its excellent resolution and high throughput, cryoSEM imaging has recently seen resurgence. In this work, we report on the development of cryogenic triple ion gun milling (CryoTIGM™), a broad ion beam milling technique for cryo-planing of vitrified, “frozen-hydrated” specimens. We find that sections prepared with CryoTIGM™ are smooth over exceptionally large areas (~700,000 µm2), and reveal ultrastructural details in similar or better quality than freeze-fractured samples.

Keywords

cryoSEMultrastructurehigh pressure freezingfreeze fracturecryo-planning
Type
Equipment and Techniques Development
Information
Microscopy and Microanalysis , Volume 21 , Supplement 6 , December 2015 , pp. 1616 - 1621
Copyright
© Microscopy Society of America 2015 

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