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Morphological Changes in the Motor Endplate and in the Belly Muscle Induced by Previous Static Stretching to the Climbing Protocol

Published online by Cambridge University Press:  23 July 2021

Gabriela K. Barbosa Open the ORCID record for Gabriela K. Barbosa [Opens in a new window] ,
Carolina dos S. Jacob Open the ORCID record for Carolina dos S. Jacob [Opens in a new window] ,
Mariana P. Rodrigues Open the ORCID record for Mariana P. Rodrigues [Opens in a new window] ,
Lara C. Rocha Open the ORCID record for Lara C. Rocha [Opens in a new window] ,
Jurandyr Pimentel Neto Open the ORCID record for Jurandyr Pimentel Neto [Opens in a new window]  and
Adriano P. Ciena Open the ORCID record for Adriano P. Ciena [Opens in a new window]
Gabriela K. Barbosa
Affiliation:
Department of Physical Activity, Laboratory of Morphology and Physical Activity – LAMAF, Institute of Biosciences (IB), São Paulo State University – UNESP, Rio Claro13506-900, SP, Brazil
Carolina dos S. Jacob
Affiliation:
Department of Physical Activity, Laboratory of Morphology and Physical Activity – LAMAF, Institute of Biosciences (IB), São Paulo State University – UNESP, Rio Claro13506-900, SP, Brazil
Mariana P. Rodrigues
Affiliation:
Department of Physical Activity, Laboratory of Morphology and Physical Activity – LAMAF, Institute of Biosciences (IB), São Paulo State University – UNESP, Rio Claro13506-900, SP, Brazil
Lara C. Rocha
Affiliation:
Department of Physical Activity, Laboratory of Morphology and Physical Activity – LAMAF, Institute of Biosciences (IB), São Paulo State University – UNESP, Rio Claro13506-900, SP, Brazil
Jurandyr Pimentel Neto
Affiliation:
Department of Physical Activity, Laboratory of Morphology and Physical Activity – LAMAF, Institute of Biosciences (IB), São Paulo State University – UNESP, Rio Claro13506-900, SP, Brazil
Adriano P. Ciena*
Affiliation:
Department of Physical Activity, Laboratory of Morphology and Physical Activity – LAMAF, Institute of Biosciences (IB), São Paulo State University – UNESP, Rio Claro13506-900, SP, Brazil
*
*Corresponding author: Adriano P. Ciena, E-mail: adriano.ciena@unesp.br
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Abstract

Static stretching provides benefits to the range of motion, modulates intramuscular connective tissue, and is incorporated into warm-up exercises. In this study, we present the effects in the motor endplate and belly muscle resulting from previous static stretching to climbing training. Twenty-four adult male Wistar rats were divided into four groups (n = 6 each): Sedentary (Sed), Climbing (Clb), Static stretching (Ss), and Static stretching prior to climbing (Ssc). The animals (Clb, Ss, and Ssc groups) were subjected to a training protocol 3×/week for 8 weeks, and the Ssc group was subjected to the Ss and Clb protocols in the same session. Samples from the animals were processed for immunostaining, histochemistry, and light microscopy. The Clb group presented a higher motor endplate; the Ss group presented no changes in the motor endplate; and the Ssc group demonstrated a higher compactness. We concluded that static stretching prior to the climbing protocol maintained the density of the motor endplate and increased the compactness of the neuromuscular junction structure. Also, there was a reduction in the myofibers’ diameter (Type I and IIa), an increase in myofibrillar densities (Type I and IIx, and total), and the reorganization of the myonuclei and the interstitium.

Keywords

cholinergic receptorsconnective tissuegastrocnemius muscleneuromuscular junctionskeletal muscle fibers
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 27 , Issue 5 , October 2021 , pp. 1183 - 1191
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

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References

Barbosa, GM, Trajano, GS, Dantas, GAF, Silva, BR & Vieira, WHB (2020). Chronic effects of static and dynamic stretching on hamstrings eccentric strength and functional performance: A randomized controlled trial. J Strength Cond Res 34, 2031–2039.CrossRefGoogle ScholarPubMed
Behm, DG, Blazevich, AJ, Kay, AD & McHugh, M (2015). Acute effects of muscle stretching on physical performance, range of motion, and injury incidence in healthy active individuals: A systematic review. Appl Physiol Nutr Metab 41, 1–11.CrossRefGoogle ScholarPubMed
Behm, DG, Kay, AD, Trajano, GS & Blazevich, AJ (2021). Mechanisms underlying performance impairments following prolonged static stretching without a comprehensive warm-up. Eur J Appl Physiol 121, 67–94.CrossRefGoogle ScholarPubMed
Bertolini, GRF, Barbieri, CH & Mazzer, N (2009). Análise longitudinal de músculos sóleos, de ratos, submetidos a alongamento passivo com uso prévio de ultrassom terapêutico. Rer Bras Med Esporte 15, 115–118.CrossRefGoogle Scholar
Bhutda, S, Surve, M, Anil, A, Kamath, K, Singh, N, Modi, D & Banerjee, A (2017). Histochemical staining of collagen and identification of Its subtypes by Picrosirius red dye in mouse reproductive tissues. Bio-Protocol 7, 1–10.CrossRefGoogle ScholarPubMed
Blazevich, AJ (2006). Effects of physical training and detraining, immobilisation, growth and aging on human fascicle geometry. Sports Med 36, 1003–1017.CrossRefGoogle ScholarPubMed
Boehm, I, Miller, J, Wishart, TM, Wigmore, SJ, Skipworth, RJE, Jones, RA & Gillingwater, TH (2020). Neuromuscular junctions are stable in patients with cancer cachexia. J Clin Invest 130, 1461–1465.CrossRefGoogle ScholarPubMed
Burden, SJ, Huijbers, MG & Remedio, L (2018). Fundamental molecules and mechanisms for forming and maintaining neuromuscular synapses. Int J Mol Sci 19, 1–19.CrossRefGoogle ScholarPubMed
Cardiff, RD, Miller, CH & Munn, RJ (2014). Manual hematoxylin and eosin staining of mouse tissue sections. Cold Spring Harb Protoc 2014(6), 655–658.CrossRefGoogle ScholarPubMed
Dayton, P (2017). Anatomic, vascular, and mechanical overview of the achilles tendon. Clin Podiatr Med Surg 34, 107–113.CrossRefGoogle ScholarPubMed
Deschenes, MR, Judelson, DA, Kraemer, WJ, Meskaitis, VJ, Volek, JS, Nindl, BC, Harman, FS & Deaver, DR (2000). Effects of resistance training on neuromuscular junction morphology. Muscle Nerve 23, 1576–1581.3.0.CO;2-J>CrossRefGoogle ScholarPubMed
Deschenes, MR, Kressin, KA, Garratt, RN, Leathrum, CM & Shaffrey, EC (2016). Effects of exercise training on neuromuscular junction morphology and pre- to post-synaptic coupling in young and aged rats. Neuroscience 316, 167–177.CrossRefGoogle Scholar
Douglas, J, Pearson, S, Ross, A & McGuigan, M (2017). Chronic adaptations to eccentric training: A systematic review. Sports Med 47, 917–941.CrossRefGoogle ScholarPubMed
Ferreira-Júnior, JB, Freitas, EDS & Chaves, SFN (2020). Exercise: A protective measure or an “open window” for COVID-19? A mini review. Front Sports Act Living 2(61), 1–6.Google ScholarPubMed
Fongy, A, Falcone, S, Lainé, J, Prudhon, B, Martins-Bach, A & Bitoun, M (2019). Nuclear defects in skeletal muscle from a dynamin 2-linked centronuclear myopathy mouse model. Sci Rep 9(1580), 1–12.CrossRefGoogle ScholarPubMed
Freitas, SR, Mendes, B, Le Sant, G, Andrade, RJ, Nordez, A & Milanovic, Z (2018). Can chronic stretching change the muscle-tendon mechanical properties? A review. Scand J Med Sci Sports 28, 794–806.CrossRefGoogle ScholarPubMed
Gianelo, MCS, Polizzelo, JC, Chesca, D & Mattiello-Sverzut, AC (2016). Three days of intermittent stretching after muscle disuse alters the proteins involved in force transmission in muscle fibers in weanling rats. Braz J Med Biol Res 49, 1–8.CrossRefGoogle ScholarPubMed
Gould, DJ, Vadakkan, TJ, Poche, RA & Dickinson, ME (2011). Multifractal and lacunarity analysis of microvascular morphology and remodeling. Microcirculation 18(2), 136–151.CrossRefGoogle ScholarPubMed
Guzzoni, V, Ribeiro, MBT, Lopes, GN, Marqueti, RdC, de Andrade, RV, Selistre-de-Araujo, HS & Durigan, JLQ (2018). Effect of resistance training on extracellular matrix adaptations in skeletal muscle of older rats. Front Physiol 9(374), 1–10.CrossRefGoogle ScholarPubMed
Hoy, KC, Strain, MM, Turtle, JD, Lee, KH, Huie, JR, Hartman, JJ, Tarbet, MM, Harlow, ML, Magnuson, DSK & Grau, JW (2020). Evidence that the central nervous system can induce a modification at the neuromuscular junction that contributes to the maintenance of a behavioral response. J Neurosci 40, 9186–9209.CrossRefGoogle Scholar
Jones, RA, Reich, CD, Dissanayake, KN, Kristmundsdottir, F, Findlater, GS, Ribchester, RR, Simmen, MW & Gillingwater, TH (2017). NMJ-morph reveals principal components of synaptic morphology influencing structure — Function relationships at the neuromuscular junction. Open Biol 6, 1–16.Google Scholar
Jorgenson, KW, Phillips, SM & Hornberger, TA (2020). Identifying the structural adaptations that drive the mechanical load-induced growth of skeletal muscle: A scoping review. Cells 9, 1–32.CrossRefGoogle ScholarPubMed
Karperien, A (2007). FracLac for ImageJ: FracLac Advanced User's Manual. Australia: Charles Sturt University, pp. 1–36. Available at https://imagej.nih.gov/ij/plugins/fraclac/fraclac.html (retrieved March 1, 2021).Google Scholar
Karperien, A, Jelinek, HF, Leandro, JJ, Soares, JV Jr, Cesar, RM & Luckie, A (2008). Automated detection of proliferative retinopathy in clinical practice. Clin Ophthalmol 2, 109–122.Google ScholarPubMed
Karperien, AL & Jelinek, HF (2015). Fractal, multifractal, and lacunarity analysis of microglia in tissue engineering. Front Bioeng Biotechnol 3(51), 1–4.CrossRefGoogle ScholarPubMed
Kikuchi, N & Nakazato, K (2017). Low-load bench press and push-up induce similar muscle hypertrophy and strength gain. J Exerc Sci Fit 15(1), 37–42.CrossRefGoogle ScholarPubMed
Ko, CP & Robitaille, R (2015). Perisynaptic schwann cells at the neuromuscular synapse: Adaptable, multitasking glial cells. Cold Spring Harbor Perspect Biol 7, 1–19.CrossRefGoogle Scholar
Korolj, A, Wu, H & Radisic, M (2019). A healthy dose of chaos: Using fractal frameworks for engineering higher- fidelity biomedical systems. Biomaterials 219, 119363.CrossRefGoogle ScholarPubMed
Kotarsky, CJ, Christensen, BK, Miller, JS & Hackney, KJ (2018). Effect of progressive calisthenic push-up training on muscle strength and thickness. J Strength Cond Res 32, 651–659.CrossRefGoogle ScholarPubMed
Krause Neto, W, Ciena, AP, Anaruma, CA, De Souza, RR & Gama, EF (2015). Effects of exercise on neuromuscular junction components across age: Systematic review of animal experimental studies neuroscience. BMC Res Notes 8, 1–15.CrossRefGoogle Scholar
Krause Neto, W, de Assis Silva, W, Ciena, AP, Anaruma, CA & Gama, EF (2017). Divergent effects of resistance training and anabolic steroid on the postsynaptic region of different skeletal muscles of aged rats. Exp Gerontol 98, 80–90.CrossRefGoogle ScholarPubMed
Krause Neto, W, Silva, WA, Polican, CA, Bocalini, D, Nucci, RAB, Anaruma, CA & Gama, EF (2018). Total training load may explain similar strength gains and muscle hypertrophy seen in aged rats submitted to resistance training and anabolic steroids. Aging Male 21, 65–76.CrossRefGoogle ScholarPubMed
Li, Y, Lee, Yi & Thompson, WJ (2011). Changes in aging mouse neuromuscular junctions are explained by degeneration and regeneration of muscle fiber segments at the synapse. J Neurosci 31, 14910–14919.CrossRefGoogle Scholar
Ma, J, Smith, BP, Smith, TL, Walker, FO, Rosencrance, EV & Koman, LA (2002). Juvenile and adult rat neuromuscular junctions: Density, distribution, and morphology. Muscle Nerve 26, 804–809.CrossRefGoogle ScholarPubMed
Masschelein, E, D'Hulst, G, Zvick, J, Hinte, L, Soro-Arnaiz, I, Gorski, T, von Meyenn, F, Bar-Nur, O & De Bock, K (2020). Exercise promotes satellite cell contribution to myofibers in a load-dependent manner. Skelet Muscle 10, 1–15.CrossRefGoogle Scholar
Metze, K, Adam, R & Florindo, JB (2019). The fractal dimension of chromatin — A potential molecular marker for carcinogenesis, tumor progression and prognosis. Expert Rev Mol Diagn 19(4), 299–312.CrossRefGoogle ScholarPubMed
Moustogiannis, A, Philippou, A, Zevolis, E, Taso, O, Chatzigeorgiou, A & Koutsilieris, M (2020). Characterization of optimal strain, frequency and duration of mechanical loading on skeletal myotubes’ biological responses. In Vivo 34, 1779–1788.CrossRefGoogle ScholarPubMed
Nishimune, H, Stanford, JA & Mori, Y (2014). Role of exercise in maintaining the integrity of the neuromuscular junction. Muscle Nerve 49, 315–324.CrossRefGoogle ScholarPubMed
Pacagnelli, FL, Sabela, AKDDA, Mariano, TB, Ozaki, GAT, Castoldi, RC, do Carmo, EM, Carvalho, RF, Tomasi, LC, Okoshi, K & Vanderlei, LCM (2016). Fractal dimension in quantifying experimental-pulmonary-hypertension-induced cardiac dysfunction in rats. Arq Bras Cardiol 107, 33–39.Google ScholarPubMed
Padilha, CS, Cella, PS, Ribeiro, AS, Voltarelli, FA, Testa, MTJ, Marinello, PC, Iarosz, KC, Guirro, PB & Deminice, R (2019). Moderate vs high-load resistance training on muscular adaptations in rats. Life Sci 238, 116964.CrossRefGoogle ScholarPubMed
Peixinho, CC, Martins, NSF, De Oliveira, LF & Machado, JC (2014). Structural adaptations of rat lateral gastrocnemius muscle-tendon complex to a chronic stretching program and their quantification based on ultrasound biomicroscopy and optical microscopic images. Clin Biomech 29, 57–62.CrossRefGoogle ScholarPubMed
Pimentel Neto, J, Rocha, LC, Barbosa, GK, Jacob, CS, Krause Neto, W, Watanabe, Is & Ciena, AP (2020). Myotendinous junction adaptations to ladder-based resistance training: Identification of a new telocyte niche. Sci Rep 10, 1–8.CrossRefGoogle ScholarPubMed
Qaisar, R, Bhaskaran, S & Van Remmen, H (2016). Muscle fiber type diversification during exercise and regeneration. Free Radical Biol Med 98, 56–67.CrossRefGoogle ScholarPubMed
Rocha, LC, Jacob, CS, Barbosa, GK, Pimentel Neto, J, Krause Neto, W, Gama, EF & Ciena, AP (2020 a). Remodeling of the skeletal muscle and postsynaptic component after short-term joint immobilization and aquatic training. Histochem Cell Biol 154, 621–628.CrossRefGoogle ScholarPubMed
Rocha, LC, Pimentel Neto, J, de Sant'Ana, JS, Jacob, CS, Barbosa, GK, Krause Neto, W, Watanabe, IS & Ciena, AP (2020b). Repercussions on sarcomeres of the myotendinous junction and the myofibrillar type adaptations in response to different trainings on vertical ladder. Microsc Res Tech, 83, 1190–1197.Google Scholar
Stankovic, M, Pantic, I, De Luka, SR, Puskas, N, Zaletel, I, Milutinovic-Smiljanic, S, Pantic, S & Trbovich, AM (2016). Quantification of structural changes in acute inflammation by fractal dimension, angular second moment and correlation. J Microsc 261, 277–284.CrossRefGoogle ScholarPubMed
Yamashita, T, Ishii, S & Oota, I (1992). Effect of muscle stretching on the activity of neuromuscular transmission. Med Sci Sports Exercise 24, 80–84.CrossRefGoogle ScholarPubMed
Zotz, TG, Capriglione, LGA, Zotz, R, Noronha, L, De Azevedo, MLV, Fiuza Martins, HR & Silveira Gomes, AR (2016). Acute effects of stretching exercise on the soleus muscle of female aged rats. Acta Histochem 118, 1–9.CrossRefGoogle ScholarPubMed