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Simultaneous Observation of Cells and Nuclear Tracks from the Boron Neutron Capture Reaction by UV-C Sensitization of Polycarbonate

Published online by Cambridge University Press:  09 July 2015

Agustina Portu ,
Andrés Eugenio Rossini ,
Silvia Inés Thorp ,
Paula Curotto ,
Emiliano César Cayetano Pozzi ,
Pablo Granell ,
Federico Golmar ,
Rómulo Luis Cabrini  and
Gisela Saint Martin
Agustina Portu*
Affiliation:
Department of Radiobiology, National Atomic Energy Commission (CNEA), Av. General Paz 1499, B1650KNA, San Martín, Buenos Aires, Argentina National Research Council (CONICET), Av. Rivadavia 1917, C1033AAJ, Ciudad Autónoma de Buenos Aires, Argentina
Andrés Eugenio Rossini
Affiliation:
Nuclear Regulatory Authority (ARN), Libertador 8250, C1429BNP, Ciudad Autónoma de Buenos Aires, Argentina
Silvia Inés Thorp
Affiliation:
Department of Instrumentation and Control, CNEA, Presbítero Juan González Aragón, B1802AYA, Ezeiza, Buenos Aires, Argentina
Paula Curotto
Affiliation:
Department of Research and Production Reactors, CNEA, Presbítero Juan González Aragón, B1802AYA, Ezeiza, Buenos Aires, Argentina
Emiliano César Cayetano Pozzi
Affiliation:
Department of Research and Production Reactors, CNEA, Presbítero Juan González Aragón, B1802AYA, Ezeiza, Buenos Aires, Argentina
Pablo Granell
Affiliation:
Micro and Nanotechnology Centre of the Bicentennial (CNMB), National Institute of Industrial Technology (INTI), Av. Gral. Paz 5445, Ed. 42, B1650JKA, San Martín, Buenos Aires, Argentina
Federico Golmar
Affiliation:
National Research Council (CONICET), Av. Rivadavia 1917, C1033AAJ, Ciudad Autónoma de Buenos Aires, Argentina Micro and Nanotechnology Centre of the Bicentennial (CNMB), National Institute of Industrial Technology (INTI), Av. Gral. Paz 5445, Ed. 42, B1650JKA, San Martín, Buenos Aires, Argentina School of Science & Technology, National University of San Martín (UNSAM), Martín de Irigoyen 3100, B1650JKA, San Martín, Buenos Aires, Argentina
Rómulo Luis Cabrini
Affiliation:
Department of Radiobiology, National Atomic Energy Commission (CNEA), Av. General Paz 1499, B1650KNA, San Martín, Buenos Aires, Argentina Faculty of Dentistry, University of Buenos Aires, Marcelo T. de Alvear 2142, C1122AAH, Ciudad Autónoma de Buenos Aires, Argentina Microspectrophotometry Laboratory (LANAIS-MEF), CONICET-CNEA, Av. General Paz 1499, B1650KNA, San Martín, Buenos Aires, Argentina
Gisela Saint Martin
Affiliation:
Department of Radiobiology, National Atomic Energy Commission (CNEA), Av. General Paz 1499, B1650KNA, San Martín, Buenos Aires, Argentina Institute of Technology “Prof. Jorge Sabato”, UNSAM, Av. General Paz 1499, B1650KNA, San Martín, Buenos Aires, Argentina
*
*Corresponding author. agustina.portu@gmail.com; portu@cnea.gov.ar
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Abstract

The distribution of boron in tissue samples coming from boron neutron capture therapy protocols can be determined through the analysis of its autoradiography image on a nuclear track detector. A more precise knowledge of boron atom location on the microscopic scale can be attained by the observation of nuclear tracks superimposed on the sample image on the detector. A method to produce an “imprint” of cells cultivated on a polycarbonate detector was developed, based on the photodegradation properties of UV-C radiation on this material. Optimal conditions to generate an appropriate monolayer of Mel-J cells incubated with boronophenylalanine were found. The best images of both cells and nuclear tracks were obtained for a neutron fluence of 1013 cm−2, 6 h UV-C (254 nm) exposure, and 4 min etching time with a KOH solution. The imprint morphology was analyzed by both light and scanning electron microscopy. Similar samples, exposed to UV-A (360 nm) revealed no cellular imprinting. Etch pits were present only inside the cell imprints, indicating a preferential boron uptake (about threefold the incubation concentration). Comparative studies of boron absorption in different cell lines and in vitro evaluation of the effect of diverse boron compounds are feasible with this methodology.

Keywords

neutron autoradiographynuclear tracksUV-CBNCTMel-Jboron imagingSEMpolycarbonate
Type
Biological Applications and Techniques
Information
Microscopy and Microanalysis , Volume 21 , Issue 4 , August 2015 , pp. 796 - 804
Copyright
© Microscopy Society of America 2015 

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