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Tracing CD34+ Stromal Fibroblasts in Palatal Mucosa and Periodontal Granulation Tissue as a Possible Cell Reservoir for Periodontal Regeneration

Published online by Cambridge University Press:  04 June 2015

Alexandra Roman ,
Emőke Páll ,
Carmen M. Mihu ,
Adrian S. Petruţiu ,
Lucian Barbu-Tudoran ,
Radu S. Câmpian ,
Adrian Florea  and
Carmen Georgiu
Alexandra Roman
Affiliation:
Department of Periodontology, Faculty of Dental Medicine, Iuliu Haţieganu University of Medicine and Pharmacy, 15 V. Babeş Street, 400012 Cluj-Napoca, Romania
Emőke Páll
Affiliation:
Department of Periodontology, Faculty of Dental Medicine, Iuliu Haţieganu University of Medicine and Pharmacy, 15 V. Babeş Street, 400012 Cluj-Napoca, Romania Department of Veterinary Reproduction, Obstetrics and Gynecology, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 3-5 Mănăştur Street, 400372 Cluj-Napoca, Romania
Carmen M. Mihu
Affiliation:
Department of Histology, Faculty of Medicine, Iuliu Haţieganu University of Medicine and Pharmacy, 6 L. Pasteur Street, 400349 Cluj-Napoca, Romania
Adrian S. Petruţiu
Affiliation:
Department of Periodontology, Faculty of Dental Medicine, Iuliu Haţieganu University of Medicine and Pharmacy, 15 V. Babeş Street, 400012 Cluj-Napoca, Romania
Lucian Barbu-Tudoran
Affiliation:
Department of Molecular Biology and Biotechnologies, Faculty of Biology and Geology, Babeş-Bolyai University, 5-7 Clinicilor Street, 400006 Cluj-Napoca, Romania
Radu S. Câmpian
Affiliation:
Department of Oral Rehabilitation, Faculty of Dental Medicine, Iuliu Haţieganu University of Medicine and Pharmacy, 15 V. Babeş Street, 400012 Cluj-Napoca, Romania
Adrian Florea*
Affiliation:
Department of Cell and Molecular Biology, Faculty of Medicine, Iuliu Haţieganu University of Medicine and Pharmacy, 6 L. Pasteur Street, 400349 Cluj-Napoca, Romania
Carmen Georgiu
Affiliation:
Department of Pathology, Faculty of Medicine, Iuliu Haţieganu University of Medicine and Pharmacy, 8 V. Babeş Street, 400012 Cluj-Napoca, Romania
*
*Corresponding author.aflorea@umfcluj.ro; adrian_a_florea@yahoo.com
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Abstract

The aim of the present research was to trace CD34+ stromal fibroblastic cells (CD34+ SFCs) in the palatal connective tissue harvested for muco-gingival surgical procedures and in granulation tissues from periodontal pockets using immunohistochemical and transmission electron microscopy. Immunohistochemical analysis targeted the presence of three antigens: CD31, α-smooth muscle actin (α-SMA), and CD34. In the palate, CD31 staining revealed a colored inner ring of the vessels representing the endothelium, α-SMA+ was located in the medial layer of the vasculature, and CD34 was intensely expressed by endothelial cells and artery adventitial cells (considered to be CD34+ SFCs). Granulation tissue showed the same pattern for CD31+ and α-SMA, but a different staining pattern for CD34. Ultrastructural examination of the palatal tissue highlighted perivascular cells with fibroblast-like characteristics and pericytes in close spatial relationship to endothelial cells. The ultrastructural evaluation of granulation tissue sections confirmed the presence of neovasculature and the inflammatory nature of this tissue. The present study traced the presence of CD34+ SFCs and of pericytes in the palatal connective tissue thus highlighting once more its intrinsic regenerative capabilities. The clinical and systemic factors triggering mobilization and influencing the fate of local CD34+SCFs and other progenitors are issues to be further investigated.

Keywords

tissue graftimmunohistochemistryultrastructureCD34 antigenpericytes
Type
Biological Applications and Techniques
Information
Microscopy and Microanalysis , Volume 21 , Issue 4 , August 2015 , pp. 837 - 848
Copyright
© Microscopy Society of America 2015 

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Footnotes

a

Roman A. and Páll E. equally contributed to the present study and can be regarded, therefore, as being main authors.

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