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Ultrastructural and Molecular Development of the Myotendinous Junction Triggered by Stretching Prior to Resistance Exercise

Published online by Cambridge University Press:  08 March 2022

Carolina dos S. Jacob Open the ORCID record for Carolina dos S. Jacob [Opens in a new window] ,
Gabriela K. Barbosa Open the ORCID record for Gabriela K. Barbosa [Opens in a new window] ,
Mariana P. Rodrigues Open the ORCID record for Mariana P. Rodrigues [Opens in a new window] ,
Jurandyr Pimentel Neto Open the ORCID record for Jurandyr Pimentel Neto [Opens in a new window] ,
Lara C. Rocha-Braga Open the ORCID record for Lara C. Rocha-Braga [Opens in a new window] ,
Camilla G. de Oliveira Open the ORCID record for Camilla G. de Oliveira [Opens in a new window] ,
Marucia Chacur Open the ORCID record for Marucia Chacur [Opens in a new window]  and
Adriano P. Ciena Open the ORCID record for Adriano P. Ciena [Opens in a new window]
Carolina dos S. Jacob
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), Institute of Biosciences, São Paulo State University (UNESP), Rio Claro 13506-900, SP, Brazil
Gabriela K. Barbosa
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), Institute of Biosciences, São Paulo State University (UNESP), Rio Claro 13506-900, SP, Brazil
Mariana P. Rodrigues
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), Institute of Biosciences, São Paulo State University (UNESP), Rio Claro 13506-900, SP, Brazil
Jurandyr Pimentel Neto
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), Institute of Biosciences, São Paulo State University (UNESP), Rio Claro 13506-900, SP, Brazil
Lara C. Rocha-Braga
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), Institute of Biosciences, São Paulo State University (UNESP), Rio Claro 13506-900, SP, Brazil
Camilla G. de Oliveira
Affiliation:
Laboratory of Functional Neuroanatomy of Pain (LAND), Department of Anatomy, Universidade de Sao Paulo (USP), São Paulo, SP, Brazil
Marucia Chacur
Affiliation:
Laboratory of Functional Neuroanatomy of Pain (LAND), Department of Anatomy, Universidade de Sao Paulo (USP), São Paulo, SP, Brazil
Adriano P. Ciena*
Affiliation:
Laboratory of Morphology and Physical Activity (LAMAF), Institute of Biosciences, São Paulo State University (UNESP), Rio Claro 13506-900, SP, Brazil
*
*Corresponding author: Adriano P. Ciena, E-mail: adriano.ciena@unesp.br
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Abstract

The myotendinous junction (MTJ) is a highly specialized region of the locomotor apparatus. Here, we investigated the ultrastructural and molecular effects in the MTJ region after static stretching prior to the ladder-based resistance training. Thirty-two male, 60-day old Wistar rats were divided into four groups: Sedentary, Resistance Training, Stretching, and Stretching-Resistance Training. The gastrocnemius muscle was processed for transmission electron microscopy techniques and Western blot assay. We observed that the static stretching prior to the ladder-based resistance training increased the MTJ components, the fibroblast growth factor (FGF)-2 and FGF-6 protein expression. Also, we demonstrated the lower transforming growth factor expression and no difference in the lysyl oxidase expression after combined training. The MTJ alterations in response to combined training demonstrate adaptive mechanisms which can be used for the prescription or development of methods to reduce or prevent injuries in humans and promote the myotendinous interface benefit.

Keywords

FGFmyotendinous interfaceresistance trainingsarcomereTGF-β1
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 28 , Issue 2 , April 2022 , pp. 537 - 542
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of the Microscopy Society of America

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Footnotes

†

These authors contributed equally to this study.

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