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Acidocalcisomes of Phytomonas françai Possess Distinct Morphological Characteristics and Contain Iron

Published online by Cambridge University Press:  01 October 2004

Kildare Miranda ,
Claudia O. Rodrigues ,
Joachim Hentchel ,
Anibal Vercesi ,
Helmut Plattner ,
Wanderley de Souza  and
Roberto Docampo
Kildare Miranda
Affiliation:
Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Av. Brigadeiro Trompovski, s/n., bloco G, Cidade Universitária, 21949-900, Rio de Janeiro, RJ, Brazil Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana–Champaign, Urbana, IL 61802
Claudia O. Rodrigues
Affiliation:
Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana–Champaign, Urbana, IL 61802
Joachim Hentchel
Affiliation:
Department of Biology, University of Konstanz, 78457 Konstanz, Germany
Anibal Vercesi
Affiliation:
Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana–Champaign, Urbana, IL 61802 Laboratório de Bioenergética, Núcleo de Medicina e Cirugia Experimental, Universidade Estadual Campinas, Campinas-SP, Brazil
Helmut Plattner
Affiliation:
Department of Biology, University of Konstanz, 78457 Konstanz, Germany
Wanderley de Souza
Affiliation:
Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Av. Brigadeiro Trompovski, s/n., bloco G, Cidade Universitária, 21949-900, Rio de Janeiro, RJ, Brazil
Roberto Docampo
Affiliation:
Laboratory of Molecular Parasitology, Department of Pathobiology and Center for Zoonoses Research, University of Illinois at Urbana–Champaign, Urbana, IL 61802
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Abstract

Acidocalcisomes are acidic calcium storage compartments described initially in trypanosomatid and apicomplexan parasites, and recently found in other unicellular eukaryotes. The aim of this study was to identify the presence of acidocalcisomes in the plant trypanosomatid Phytomonas françai. Electron-dense organelles of P. françai were shown to contain large amounts of oxygen, sodium, magnesium, phosphorus, potassium, calcium, iron, and zinc as determined by X-ray microanalysis, either in situ or when purified using iodixanol gradient centrifugation or by elemental mapping. The presence of iron is not common in other acidocalcisomes. In situ, but not when purified, these organelles showed an elongated shape differing from previously described acidocalcisomes. However, these organelles also possessed a vacuolar H+-pyrophosphatase (V-H+-PPase) as determined by biochemical methods and by immunofluorescence microscopy using antibodies against the enzyme. Together, these results suggest that the electron-dense organelles of P. françai are homologous to the acidocalcisomes described in other trypanosomatids, although with distinct morphology and elemental content.

Keywords

Phytomonasacidocalcisomeselemental mappingpyrophosphatasecalciumtransmission electron microscopy
Type
Feature Articles
Information
Microscopy and Microanalysis , Volume 10 , Issue 5 , October 2004 , pp. 647 - 655
Copyright
© 2004 Microscopy Society of America

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References

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