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Alterations in Cardiac Structure and Function in a Murine Model of Chronic Alcohol Consumption

Published online by Cambridge University Press:  09 May 2012

Brittany A. Law ,
Scott P. Levick  and
Wayne E. Carver
Brittany A. Law*
Affiliation:
Cell Biology and Anatomy, School of Medicine, University of South Carolina, Columbia, SC 29208, USA
Scott P. Levick
Affiliation:
Cell Biology and Anatomy, School of Medicine, University of South Carolina, Columbia, SC 29208, USA
Wayne E. Carver
Affiliation:
Cell Biology and Anatomy, School of Medicine, University of South Carolina, Columbia, SC 29208, USA
*
Corresponding author. E-mail: Brittany.Law@uscmed.sc.edu
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Abstract

Male, wild-type, FVB strain mice were fed a nutritionally complete liquid diet supplemented with 4% ethanol v/v over a time course of 1, 2, 4, 8, 12, and 14 weeks. Controls were offered an isocaloric liquid equivalent and pair fed with their ethanol counterparts. Changes in cardiac physiology were assessed at respective time points via echocardiography. Additionally, the use of histological techniques, mRNA analysis, apoptosis determination, and immunohistochemistry were employed to determine the functional and structural changes on the heart. Echocardiograph analysis revealed a compensatory phase that occurred early in the time course (1–8 weeks) and decompensation reverting toward heart failure at weeks 12 and 14. Throughout the study, an increase in cardiomyocyte hypertrophy, cardiac fibrosis, apoptosis, TGF-β, and the presence of α-SMA-positive cells were determined. A compensatory period in mice treated with ethanol occurred early followed by a transition to a dilated phenotype over time. A number of factors may be involved in this process including the activation of myofibroblasts and their fibrotic activities that is correlated with the presence of transforming growth factor beta.

Keywords

alcoholic cardiomyopathymyofibroblastsfibrosishypertrophytransforming growth factor betaalpha smooth muscle actin
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 18 , Issue 3 , June 2012 , pp. 453 - 461
Copyright
Copyright © Microscopy Society of America 2012

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