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Alterations in the Molecular Interaction of Bcl-2 and Bax During Apoptosis Assessed Using Fluroescence Resonance Energy Transfer (FRET) Microscopy and Green Fluorescent Protein (GFP)-Bax and Blue Fluorescent Protein (BFP)-Bcl-2 Expressing Proteins

Published online by Cambridge University Press:  02 July 2020

N. Mahajan  and
B. Herman
N. Mahajan
Affiliation:
Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill, Chapel Hill, NC27599
B. Herman
Affiliation:
Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill, Chapel Hill, NC27599
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Extract

Apoptosis or programmed cell death, is a mechanism of cellular demise which is believed to play a role in many physiological processes, and which when defective, can contribute to the pathogenesis of cancer. Apoptosis is stimulated in cells by the p53 tumor suppresser, Bax and El A proteins and inhibited by overexpression of Bcl-2 and adenovirus E1B 19 kDa oncoproteins. A role for altered apoptosis in cancer comes from recent reports which have documented that inactivation of p53 (which promotes apoptosis) and/or overexpression of bcl-2 (which inhibits apoptosis) occurs in numerous cancers. Recent work from our laboratory has shown that inactivation of p53 (either by physical complex formation between p53 and Human Papillomavirus (HPV) type 16- or 18- E6 protein or by mutation of p53), is associated with increased expression of bcl-2 in cervical carcinoma cells. The combination of inactive p53 and increased expression of bcl-2 may lead to inhibition of apoptosis and thereby contribute to the development or progression of cervical cancer.

Type
Cell Biology Applications of Green Fluorescent Protein and other Vital Labeling Probes
Information
Microscopy and Microanalysis , Volume 3 , Issue S2: Proceedings: Microscopy & Microanalysis '97, Microscopy Society of America 55th Annual Meeting, Microbeam Analysis Society 31st Annual Meeting, Histochemical Society 48th Annual Meeting, Cleveland, Ohio, August 10-14, 1997 , August 1997 , pp. 135 - 136
Copyright
Copyright © Microscopy Society of America 1997

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