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Amino Acid Composition and Immunolabelling of a 42.5 KDA Protein from Phloem Exudate of Luffa Cylindrica Fruits

Published online by Cambridge University Press:  02 July 2020

M. C. Wang  and
Y. R. Chen
M. C. Wang
Affiliation:
Department of Botany, National Taiwan University, Taipei, Taiwan
Y. R. Chen
Affiliation:
Department of Botany, National Taiwan University, Taipei, Taiwan
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Extract

Phloem proteins are specifically presented in phloem tissues of monocotyledons and dicotyledons. Ultrastructural studies showed that they were filamentous, spherical, tubular, crystalline and amorphous in shaped They were present in the sieve elements and sometimes are also found in companion cells and phloem parenchyma cells. The diversities in shape and biogenesis of plant p-protein in relation to plant species and developmental stage of phloem tissues were reported. In the present study, a 42.5 kDA protein obtained from phloem exudate of Luffa cylindrica fruits were examined with amino acid composition, immnoblotting, and immunolocalization.

Phloem exudates leaking from Luffa cylindrica fruits by a simple razor blade cutting were mixed with the extraction medium containing antioxidant and rapidly separated by 2-dimensional gel of native and sodium dodecylsulfate (SDS) polyacrylamide gel electrophoresis (PAGE).

Type
Botany/Plant Pathology
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1184 - 1186
Copyright
Copyright © Microscopy Society of America

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