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An Antibody-Sensitized Microfabricated Cantilever for the Growth Detection of Aspergillus niger Spores

Published online by Cambridge University Press:  18 January 2007

Natalia Nugaeva ,
Karin Y. Gfeller ,
Natalia Backmann ,
Marcel Düggelin ,
Hans Peter Lang ,
Hans-Joachim Güntherodt  and
Martin Hegner
Natalia Nugaeva
Affiliation:
Institute of Physics, University of Basel, Klingelbergstrasse 82, CH-4056 Basel, Switzerland
Karin Y. Gfeller
Affiliation:
Institute of Physics, University of Basel, Klingelbergstrasse 82, CH-4056 Basel, Switzerland
Natalia Backmann
Affiliation:
Institute of Physics, University of Basel, Klingelbergstrasse 82, CH-4056 Basel, Switzerland
Marcel Düggelin
Affiliation:
Pharmacentre, Microscopy Centre, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland
Hans Peter Lang
Affiliation:
Institute of Physics, University of Basel, Klingelbergstrasse 82, CH-4056 Basel, Switzerland
Hans-Joachim Güntherodt
Affiliation:
Institute of Physics, University of Basel, Klingelbergstrasse 82, CH-4056 Basel, Switzerland
Martin Hegner
Affiliation:
Institute of Physics, University of Basel, Klingelbergstrasse 82, CH-4056 Basel, Switzerland
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Abstract

We demonstrate a new sensitive biosensor for detection of vital fungal spores of Aspergillus niger. The biosensor is based on silicon microfabricated cantilever arrays operated in dynamic mode. The change in resonance frequency of the sensor is a function of mass binding to the cantilever surface. For specific A. niger spore immobilization on the cantilever, each cantilever was individually coated with anti-Aspergillus niger polyclonal antibodies. We demonstrate the detection of single A. niger spores and their subsequent growth on the functionalized cantilever surface by online measurements of resonance frequency shifts. The new biosensor operating in humid air allows quantitative and qualitative detection of A. niger spores as well as detection of vital, functional spores in situ within ∼4 h. The detection limit of the sensor is 103 CFU mL−1. Mass sensitivity of the cantilever sensor is ∼53 pg Hz−1.

Keywords

biosensormicrofabricated cantilever arrayfast fungal growth detectionanti-Aspergillus niger polyclonal antibodies
Type
Research Article
Information
Microscopy and Microanalysis , Volume 13 , Issue 1: Special Issue: Environmental, Industrial, and AppliedMicrobiology , February 2007 , pp. 13 - 17
Copyright
2007 Microscopy Society of America

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References

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