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Analysis of Orientations of Collagen Fibers by Novel Fiber-Tracking Software

Published online by Cambridge University Press:  21 November 2003

Jun Wu ,
Bartłomiej Rajwa ,
David L. Filmer ,
Christoph M. Hoffmann ,
Bo Yuan ,
Ching-Shoei Chiang ,
Jennie Sturgis  and
J. Paul Robinson
Jun Wu
Affiliation:
Department of Biology, Purdue University, West Lafayette, IN 47907, USA
Bartłomiej Rajwa
Affiliation:
Department of Basic Medical Sciences, Purdue University, West Lafayette, IN 47907, USA Department of Biophysics, The Jan Zurzycki Institute of Molecular Biology and Biotechnology, Jagiellonian University, ul. Gronostajowa 7, 30-387 Kraków, Poland
David L. Filmer
Affiliation:
Department of Biology, Purdue University, West Lafayette, IN 47907, USA
Christoph M. Hoffmann
Affiliation:
Department of Computer Science, Purdue University, West Lafayette, IN 47907, USA
Bo Yuan
Affiliation:
Department of Computer Science, Purdue University, West Lafayette, IN 47907, USA
Ching-Shoei Chiang
Affiliation:
Department of Computer Science, Purdue University, West Lafayette, IN 47907, USA
Jennie Sturgis
Affiliation:
Department of Basic Medical Sciences, Purdue University, West Lafayette, IN 47907, USA
J. Paul Robinson
Affiliation:
Department of Basic Medical Sciences, Purdue University, West Lafayette, IN 47907, USA Department of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA
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Abstract

Recent evidence supports the notion that biological functions of extracellular matrix (ECM) are highly correlated to not only its composition but also its structure. This article integrates confocal microscopy imaging and image-processing techniques to analyze the microstructural properties of ECM. This report describes a two- and three-dimensional fiber middle-line tracing algorithm that may be used to quantify collagen fibril organization. We utilized computer simulation and statistical analysis to validate the developed algorithm. These algorithms were applied to confocal images of collagen gels made with reconstituted bovine collagen type I, to demonstrate the computation of orientations of individual fibers.

Keywords

image processingconfocal microscopyECMcollagen fibersorientation
Type
Microscopy Techniques
Information
Microscopy and Microanalysis , Volume 9 , Issue 6 , December 2003 , pp. 574 - 580
Copyright
© 2003 Microscopy Society of America

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References

REFERENCES

Adams, C. & Fiona, M.W. (1993). Regulation of development and differentiation by the extracellular matrix. Development 117, 1183–1198.Google Scholar
Aumailley, M. & Gayraud, B. (1998). Structure and biological activity of the extracellular matrix. J Mol Med 76, 253–265.CrossRefGoogle Scholar
Berthiaume, F., Moghe, P.V., Toner, M., & Yarmush, M.L. (1996). Effect of extracellular matrix topology on cell structure, function, and physiological responsiveness: Hepatocytes cultured in a sandwich configuration. FASEB J 10, 1471–1484.Google Scholar
Birk, D.E., Nurminskaya, M.V., & Zycband, E.I. (1995). Collagen fibrillogenesis in situ: fibril segments undergo post-depositional modifications resulting in linear and lateral growth during matrix development. Dev Dynam 202, 229–243.CrossRefGoogle Scholar
Birk, D.E., Zycband, E.I., Woodruff, S., Winkelmann, D.A., & Trelstad, R.L. (1997). Collagen fibrillogenesis in situ: Fibril segments become long fibrils as the developing tendon matures. Dev Dynam 208, 291–298.3.0.CO;2-D>CrossRefGoogle Scholar
Bissell, M.J., Hall, H.G., & Parry, G. (1982). How does the extracellular matrix direct gene expression? J Theor Biol 99, 31–68.Google Scholar
Brightman, A.O., Rajwa, B.P., Sturgis, J.E., McCallister, M.E., Robinson, J.P., & Voytik-Harbin, S.L. (2000). Time-lapse confocal reflection microscopy of collagen fibrillogenesis and extracellular matrix assembly in vitro. Biopolymers 54, 222–234.3.0.CO;2-K>CrossRefGoogle Scholar
Chen, C.S., Mrksich, M., Huang, S., Whitesides, G.M., & Ingber, D.E. (1997). Geometric control of cell life and death. Science 276, 1425–1428.CrossRefGoogle Scholar
Choquet, D., Felsenfeld, D.P., & Sheetz, M.P. (1997). Extracellular matrix rigidity causes strengthening of integrin-cytoskeleton linkages. Cell 88, 39–48.CrossRefGoogle Scholar
Contard, P., Jacobs, L., Perlish, J.S., & Fleischmajer, R. (1993). Collagen fibrillogenesis in a three-dimensional fibroblast cell culture system. Cell Tissue Res 273, 571–575.CrossRefGoogle Scholar
Curtis, A.S. & Wilkinson, C.D. (1998). Reactions of cells to topography. J Biomat Sci—Polym E 9, 1313–1329.CrossRefGoogle Scholar
Dickinson, R.B., Guido, S., & Tranquillo, R.T. (1994). Biased cell migration of fibroblasts exhibiting contact guidance in oriented collagen gels. Ann Biomed Eng 22, 342–356.CrossRefGoogle Scholar
Friedl, P. & Brocker, E.B. (2000). The biology of cell locomotion within three-dimensional extracellular matrix. Cell Mol Life Sci 57, 41–64.CrossRefGoogle Scholar
Gunzer, M., Friedl, P., Niggemann, B., Brocker, E.B., Kampgen, E., & Zanker, K.S. (2000). Migration of dendritic cells within 3-D collagen lattices is dependent on tissue origin, state of maturation, and matrix structure and is maintained by proinflammatory cytokines. J Leukocyte Biol 67, 622–629.Google Scholar
Kadler, K.E., Holmes, D.F., Trotter, J.A., & Chapman, J.A. (1996). Collagen fibril formation. Biochem J 316, 1–11.Google Scholar
Kleinman, H.K., Klebe, R.J., & Martin, G.R. (1981). Role of collagenous matrices in the adhesion and growth of cells. J Cell Biol 88, 473–485.CrossRefGoogle Scholar
Krucinska, I. (1999a). Evaluating fibrous architecture of nonwovens with computer-assisted microscopy. Text Res J 69, 363–369.Google Scholar
Krucinska, I. (1999b). Evaluation of fibre orientation in fibrous materials. Fibres Text East Eur 7, 45–50.Google Scholar
Krucinska, S., Krucinska, I., Veeravanallur, S., & Slot, K. (1997). Computer-assisted analysis of the extracellular matrix of connective tissue. SPIE Proc 3034, 950–962.CrossRefGoogle Scholar
Lo, C.M., Wang, H.B., Dembo, M., & Wang, Y.L. (2000). Cell movement is guided by the rigidity of the substrate. Biophys J 79, 144–152.CrossRefGoogle Scholar
Ottani, V., Raspanti, M., & Ruggeri, A. (2001). Collagen structure and functional implications. Micron 32, 251–260.CrossRefGoogle Scholar
Pelham, R.J., Jr. & Wang, Y.L. (1997). Cell locomotion and focal adhesions are regulated by substrate flexibility. Proc Natl Acad Sci USA 94, 13661–13665.CrossRefGoogle Scholar
Pourdeyhimi, B., Ramanathan, R., & Dent, R. (1996a). Measuring fiber orientation in nonwovens, part I: Simulation. Text Res J 66, 713–722.Google Scholar
Pourdeyhimi, B., Ramanathan, R., & Dent, R. (1996b). Measuring fiber orientation in nonwovens, part II: Direct tracking. Text Res J 66, 747–753.Google Scholar
Pourdeyhimi, B., Ramanathan, R., & Dent, R. (1997a). Measuring fiber orientation in nonwovens, part III: Fourier transform. Text Res J 67, 143–151.Google Scholar
Pourdeyhimi, B., Ramanathan, R., & Dent, R. (1997b). Measuring fiber orientation in nonwovens, part IV: Flow field analysis. Text Res J 67, 181–190.Google Scholar
Pourdeyhimi, B., Ramanathan, R., & Dent, R. (1999). Measuring fiber orientation in nonwovens, part V: Real webs. Text Res J 69, 185–192.CrossRefGoogle Scholar
Roeder, B.A., Kokini, K., Sturgis, J.E., Robinson, J.P., & Voytik-Harbin, S.L. (2002). Tensile mechanical properties of three-dimensional type I collagen extracellular matrices with varied macrostructure. J Biomech Eng 124, 214–222.CrossRefGoogle Scholar
Russ, J.C. (1999). The Image Processing Handbook. Boca Raton, FL: CRC Press.
Sheppard, C.J.R. & Shotton, D.M. (1997). Confocal Laser Scanning Microscopy. Oxford, UK: BIOS Scientific Publishers Ltd.
Stepien, E., Stanisz, J., & Korohoda, W. (1999). Contact guidance of chick embryo neurons on single scratches in glass and on underlying aligned human skin fibroblasts. Cell Biol Int 23, 105–116.CrossRefGoogle Scholar
Voytik-Harbin, S.L., Rajwa, B., & Robinson, J.P. (2001). Three-dimensional imaging of extracellular matrix and extracellular matrix-cell interactions. Method Cell Biol 63, 583–597.CrossRefGoogle Scholar
Wang, H.B., Dembo, M., & Wang, Y.L. (2000). Substrate flexibility regulates growth and apoptosis of normal but not transformed cells. Am J Physiol—Cell Ph 279, C1345–C1350.CrossRefGoogle Scholar
Wu, J. (2002). Ph.D. thesis. Three Dimensional Modeling of Engineered Extracellular Matrix Derived from Collagen. West Lafayette, IN: Purdue University.
Yamada, K.M. (1983). Cell surface interactions with extracellular materials. Annu Rev Biochem 52, 761–799.CrossRefGoogle Scholar