Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-28T02:26:26.075Z Has data issue: false hasContentIssue false
  • English
  • Français

Anatomical, Phytochemical, and Histochemical Study of Juniperus rigida Needles at Different Altitudes

Published online by Cambridge University Press:  27 August 2019

Shun Kuang ,
Linfang Liu ,
Mingliang Qing ,
Yujia Zhang ,
Xueping Feng ,
Dongmei Wang ,
Yun Jiang ,
Xin Zhang  and
Dengwu Li
Shun Kuang
Affiliation:
College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China
Linfang Liu
Affiliation:
College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China
Mingliang Qing
Affiliation:
College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China
Yujia Zhang
Affiliation:
College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China
Xueping Feng
Affiliation:
College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China
Dongmei Wang
Affiliation:
College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China
Yun Jiang*
Affiliation:
College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China
Xin Zhang*
Affiliation:
College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China
Dengwu Li*
Affiliation:
College of Forestry, Northwest A&F University, Yangling, Shaanxi 712100, China
*
*Authors for correspondence: Yun Jiang, E-mail: 27336785@qq.com; Xin Zhang, E-mail: xin.zhang@nwsuaf.edu.cn; and Dengwu Li, E-mail: dengwuli@163.com
*Authors for correspondence: Yun Jiang, E-mail: 27336785@qq.com; Xin Zhang, E-mail: xin.zhang@nwsuaf.edu.cn; and Dengwu Li, E-mail: dengwuli@163.com
*Authors for correspondence: Yun Jiang, E-mail: 27336785@qq.com; Xin Zhang, E-mail: xin.zhang@nwsuaf.edu.cn; and Dengwu Li, E-mail: dengwuli@163.com
Get access

Abstract

Needles of Juniperus rigida are used in Chinese traditional medicine for the treatment of brucellosis, dropsy, skin disease, and rheumatoid arthritis. This is the first study that reports anatomical structures of the J. rigida needles collected at different altitudes. The most common anatomical, phytochemical, and histochemical techniques and methods are used. The results show that anatomical structures and chemical composition change significantly at different altitudes. The main anatomical characters are significant xeromorphic structures (thick epidermis, hypodermis, and cuticle), a stomatal band, a developed vascular bundle, and a marginal resin duct. The xeromorphic structures become more pronounced with increasing altitude. The phytochemical and histochemical results demonstrate that the content of the main chemical compounds (phenols and terpenoids) basically increases at a higher elevation. Histochemical analysis localizes the phenols in epidermal cells, sponge tissue, endothelial layer cells, and stomatal bands, and the terpenoids in palisade tissue, sponge tissue, and the edge of the resin duct. This work reveals the relation between anatomy and chemistry in J. rigida needles, contributes to the quality control of its ethno-medicine, and provides the evidence to develop the commercial cultivation.

Keywords

altitudeanatomyhistochemistryJuniperus rigida needlesphytochemistry
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 25 , Issue 5 , October 2019 , pp. 1213 - 1223
Copyright
Copyright © Microscopy Society of America 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Agudelo, I, Cogoi, L, Filip, R, Kuzmanich, N, Wagner, ML & Ricco, RA (2018). Anatomy, histochemistry, and comparative analysis of hydroxycinnamic derivatives in healthy leaves and galls induced by Baccharopelma spp. (Hemiptera: Psyllidae) in Baccharis spicata, (Lam) Baill (Asteraceae). Biochem Syst Ecol 77, 22–30.Google Scholar
Arrivabene, HP, Souza, IDC, Co, WLO, Conti, MM, Wunderlin, DA & Milanez, CRD (2015). Effect of pollution by particulate iron on the morphoanatomy, histochemistry, and bioaccumulation of three mangrove plant species in Brazil. Chemosphere 127, 27–34.Google Scholar
Bercu, R, Broasca, L & Popoviciu, R (2010). Comparative anatomical study of some gymnospermae species leaves. Botanica Serbica 34, 21–28.Google Scholar
Brusotti, G, Cesari, I, Gilardoni, G, Tosi, S, Grisoli, P, Picco, AM & Caccialanza, G (2012). Chemical composition and antimicrobial activity of Phyllanthus muellerianus (Kuntze) Excel essential oil. J Ethnopharmacol 142, 657–662.Google Scholar
Chauhan, RS, Tava, A, Mella, M, Nautiyal, MC & Cecotti, R (2016). Variation in the essential oil composition of Angelica archangelica from three different altitudes in Western Himalaya, India. Ind Crops Prod 94, 401–404.Google Scholar
Coelho, VPDM, Leite, JPV, Nunes, LG & Ventrella, MC (2012). Anatomy, histochemistry and phytochemical profile of leaf and stem bark of Bathysa cuspidata (Rubiaceae). Aust J Bot 60, 49–60.Google Scholar
De Souza, DMF, Sã, RD, Araújo, EL & Randau, KP (2017). Anatomical, phytochemical and histochemical study of Solidago chilensis Meyen. An Acad Bras Cienc 90, 2017–2020.Google Scholar
Eyberger, AL, Dondapati, R & Porter, JR (2006). Endophyte fungal isolates from Podophyllum peltatum produce podophyllotoxin. J Nat Prod 69, 1121–1124.Google Scholar
Gordien, AY, Gray, AI, Franzblau, SG & Seidel, V (2009). Antimycobacterial terpenoids from Juniperus communis L. (Cuppressaceae). J Ethnopharmacol 126, 500–505.Google Scholar
Hammond, KA, Szewczak, J & Król, E (2001). Effects of altitude and temperature on organ phenotypic plasticity along an altitudinal gradient. J Exp Biol 204, 1991–2000.Google Scholar
Hegedús, L, Wittmann, M, Noszticzius, Z, Yan, S, Sirimungkala, A, Försterling, HD & Field, RJ (2002). HPLC analysis of complete BZ systems. Evolution of the chemical composition in cerium and ferroin catalysed batch oscillators: Experiments and model calculations. Faraday Discuss 120, 21–38.Google Scholar
Huber, DPW, Philippe, RN, Madilao, LL, Sturrock, RN & Bohlmann, J (2005). Changes in anatomy and terpene chemistry in roots of Douglas-fir seedlings following treatment with methyl jasmonate. Tree Physiol 25, 1075–1083.Google Scholar
Ibañez, J & Usubillaga, A (2010). Analysis of the essential oil of two different altitudinal populations of Coespeletia moritziana (Sch. Bip.ex Wedd) Cuatrec. Flavour Frag J 21, 760–763.Google Scholar
Inan, M & Tel, AZ (2014). Determination of Cydotrichium niveum essential oil and its components at different altitudes. Not Bot Horti Agrobo 42, 128–131.Google Scholar
Jeong, EJ, Seo, H, Yang, H, Kim, J, Sung, SH & Kim, YC (2012). Anti-inflammatory phenolics isolated from Juniperus rigida leaves and twigs in lipopolysaccharide-stimulated RAW264.7 macrophage cells. J Enzyme Inhib Med Chem 27, 875–879.Google Scholar
Johansen, D (1940). Plant Microtechnique. New York, NY: McGraw Hill Book.Google Scholar
Kofidis, G, Bosabalidis, AM & Moustakas, M (2003). Contemporary seasonal and altitudinal variations of leaf structural features in Oregano (Origanum vulgare L.). Ann Bot 92, 635–645.Google Scholar
Konarska, A (2018). Microstructural and histochemical characteristics of Lycium barbarum, L. fruits used in folk herbal medicine and as functional food. Protoplasma 255, 1839–1854.Google Scholar
Körner, C (2007). The use of “altitude” in ecological research. Trends Ecol Evol 22, 569–574.Google Scholar
Lee, YS, Kim, JH, Kim, HJ, Sohn, EJ, Kim, CS, Jeong, IH, Jo, KH, Kim, JH & Kim, JS (2010). Anti-lipase and lipolytic activities of EtOH extract from Juniperus rigida. Korean J Pharmacogn 41, 216–220.Google Scholar
Lesjak, MM, Beara, IN, Orčić, DZ, Petar, KN, Simin, ND, Emilija, SD & Mimica-Dukic, NM (2014). Phytochemical composition and antioxidant, anti-inflammatory and antimicrobial activities of Juniperus macrocarpa, Sibth. et Sm. J Funct Foods 7, 257–268.Google Scholar
Li, FL & Bao, WK (2014). Elevational trends in leaf size of Campylotropis polyantha in the arid Minjiang River valley, SW China. J Arid Environ 108, 1–9.Google Scholar
Liu, Q, Li, D, Wang, W, Wang, D, Meng, X & Wang, Y (2016). Chemical composition and antioxidant activity of essential oils and methanol extracts of different parts from Juniperus rigida Siebold & Zucc. Chem Biodivers 13, 1240–1250.Google Scholar
Liu, Z, Wang, D, Li, D & Zhang, S (2017). Quality Evaluation of Juniperus rigida Sieb. et Zucc. based on phenolic profiles, bioactivity, and HPLC fingerprint combined with chemometrics. Front Pharmacol 8, 198.Google Scholar
Mankyu, H & Hongwook, H (2000). Genetic diversity and population structure of Juniperus rigida (Cupressaceae) and Juniperus coreana. Evol Ecol 14, 87–98.Google Scholar
Medini, H, Elaissi, A, Larbi, KM, Piras, A, Porcedda, S & Falconieri, D (2011). Chemical composition and antioxidant activity of the essential oil of Juniperus phoenicea L. berries. Nat Prod Res 25, 1695–1706.Google Scholar
Meng, X, Li, D, Zhou, D, Wang, D, Liu, Q & Fan, S (2016). Chemical composition, antibacterial activity and related mechanism of the essential oil from the leaves of Juniperus rigida Sieb. et Zucc against Klebsiella pneumoniae. J Ethnopharmacol 194, 698–705.Google Scholar
Mercadantesimões, MO, Mazzottinidossantos, HC, Nery, LA, Ferreira, PR, Ribeiro, LM & Royo, VA (2014). Structure, histochemistry and phytochemical profile of the bark of the sobol and aerial stem of Tontelea micrantha (Celastraceae—Hippocrateoideae). An Acad Bras De Cienc 86, 1167–1179.Google Scholar
Olthof, MR, Hollman, PCH & Katan, MB (2001). Chlorogenic acid and caffeic acid are absorbed in humans. J Nutr 131, 66–71.Google Scholar
Omar, FE, El-Achi, N, Bakkour, Y & El-Nakat, H (2014). HPLC analysis for identification and quantification of phenolic acids and flavonoids in Juniperus excelsa. J Nat Prod 7, 162–167.Google Scholar
Pal, M, Mishra, T, Kumar, A, Baleshwar, DK, Upreti, TS & Rana, (2015). Chemical constituents and antimicrobial potential of essential oil from Betula utilis growing in high altitude of Himalaya (India). J Essent Oil Bear Plants 18, 1078–1082.Google Scholar
Pant, KS, Kumar, D & Gairola, S (2006). Seed oil content variation in Jatropha curcas Linn. in different altitudinal ranges and site conditions in H.P. India. Lyonia 11, 31–34.Google Scholar
Parkhurst, DF & Loucks, OL (1972). Optimal leaf size in relation to environment. J Ecol 60, 505–539.Google Scholar
Perera, WH, Bizzo, HR, Gama, PE, Alviano, CS, Salimena, FRG, Alviano, DS & Leitao, SG (2017). Essential oil constituents from high altitude Brazilian species with antimicrobial activity: Baccharis parvidentata Malag. Hyptis monticola Mart. ex Benth. and Lippia origanoides Kunth. J Essent Oil Res 29, 109–116.Google Scholar
Pyankov, VI, Voznesenskaya, EV, Kondratschuk, AV & Black, CC (1997). A comparative anatomical and biochemical analysis in Salsola (Chenopodiaceae) species with and without a Kranz type leaf anatomy: A possible reversion of C4 to C3 photosynthesis. Am J Bot 84, 597–606.Google Scholar
Rangel, ML, Guerrero-Analco, JA, Monribot-Villanueva, JL, Kiel-Martinez, AL, Avendano-Reyes, S, Abad, JPD, Bonilla-Landa, I, Davalos-Sotelo, R, Olivares-Romero, JL & Angeles, G (2018). Anatomical and chemical characteristics of leaves and branches of, Juniperus deppeana, var. deppeana (Cupressaceae): A potential source of raw materials for the perfume and sweet candies industries. Ind Crop Prod 113, 50–54.Google Scholar
Rosenzweig, MR, Krech, D, Bennett, EL & Diamond, MC (1960). Effects of environmental complexity and training on brain chemistry and anatomy: A replication and extension. J Comp Physiol Psychol 55, 429–432.Google Scholar
Ryu, YB, Jeong, HJ, Kim, JH, Kim, YM, Park, JY & Kim, D (2010). Biflavonoids from Torreya nucifera displaying SARS-CoV 3CL(pro) inhibition. Bioorgan Med Chem 18, 7940–7950.Google Scholar
Said, SA, Fernandez, C, Stéphane, G, Said, SA, Fernandez, C, Greff, S, Torre, F, Derridj, A, Gauquelin, T & Mevy, JP (2011). Inter-population variability of terpenoid composition in leaves of Pistacia lentiscus L. from Algeria: A chemoecological approach. Molecules 16, 2646–2657.Google Scholar
Satil, F & Selvi, S (2007). An anatomical and ecological study of some Crocus L. taxa (Iridaceae) from the west part of Turkey. Acta Bot Croat 66, 25–33.Google Scholar
Shields, LM (1950). Leaf xeromorphy as related to physiological and structural influences. Bot Rev 16, 399–447.Google Scholar
Singh, B & Todaria, NP (2012). Nutrients composition changes in leaves of Quercus semecarpifolia at different seasons and altitudes. Ann For Res 55, 189–196.Google Scholar
Singh, R, Ahluwalia, V, Singh, P, Kumar, N, Sati, OP & Sati, N (2016). Antifungal and phytotoxic activity of essential oil from root of Senecio amplexicaulis Kunth. (Asteraceae) growing wild in high altitude-Himalayan region. Nat Prod Res 30, 1875–1879.Google Scholar
Vaiciulyte, V & Loziene, K (2013). Variation of chemical and morphological characters of leaves and unripe cones in Juniperus communis. Bot Lith 19, 37–47.Google Scholar
Vasić, P, Krivošej, Z, Topuzović, M, Dubak, D & Prodanović, D (2014). Morphological-anatomical characteristics of two common Junipers (Juniperus communis and Juniperus oxycedrus) from the area of mountain Kopaonik in Serbia. Turk J Agric For 60, 91–104.Google Scholar