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Bee Venom Induced In Vivo Ultrastructural Reactions of Cells Involved in the Bone Marrow Erythropoiesis and of Circulating Red Blood Cells

Published online by Cambridge University Press:  04 February 2013

Adrian Florea  and
Constantin Crăciun
Adrian Florea*
Affiliation:
Department of Cell and Molecular Biology, “Iuliu Hațieganu”University of Medicine and Pharmacy, 6 Pasteur St., 400349, Cluj-Napoca, Romania
Constantin Crăciun
Affiliation:
Electron Microscopy Center, “Babeş-Bolyai” University, 5-7 Clinicilor St., 400006, Cluj-Napoca, Romania
*
*Corresponding author. E-mail: aflorea@umfcluj.ro; adrian_a_florea@yahoo.com
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Abstract

Ultrastructural answer of bone marrow erythroid series and of red blood cells (RBCs) in Wistar rats to bee venom (BV) was analyzed by transmission and scanning electron microscopy, and corroborated with hematological data. A 5-day and a 30-day treatment with daily doses of 700 μg BV/kg and an acute-lethal treatment with a single dose of 62 mg BV/kg were performed. The 5-day treatment resulted in a reduced cellularity of the bone marrow, with necrosed proerythroblasts, polymorphous erythroblasts, and reticulocytes with cytoplasmic extensions, and a lower number of larger RBCs, with poikilocytosis (acanthocytosis) and anisocytosis, and reduced concentrations of hemoglobin. After the 30-day treatment, the bone marrow architecture was restored, but polymorphous erythroblasts and reticulocytes with thin extensions could still be observed, while the RBCs in higher number were smaller, many with abnormal shapes, especially acanthocytes. The acute treatment produced a partial depopulation of the bone marrow and ultrastructural changes of erythroblasts including abnormal mitochondrial cristae. The RBCs in lower number were bigger and crenated, with reduced concentrations of hemoglobin. Overall, BV was able to promote stress erythropoiesis in a time- and dose-related manner, mitochondrial cristae modification being a critical factor involved in the toxicity of the BV high doses.

Keywords

erythropoiesisbone marrowbee venomerythroblastsmitochondriaultrastructureRBCspoikilocytosis
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 2 , April 2013 , pp. 393 - 405
Copyright
Copyright © Microscopy Society of America 2013

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