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Calcaneal Tendon Collagen Fiber Morphometry and Aging

Published online by Cambridge University Press:  20 September 2017

Daniel Hadraba Open the ORCID record for Daniel Hadraba [Opens in a new window] ,
Jiri Janacek ,
Eva Filova ,
Frantisek Lopot ,
Rik Paesen ,
Ondrej Fanta ,
Anneliese Jarman ,
Alois Necas ,
Marcel Ameloot  and
Karel Jelen
Daniel Hadraba*
Affiliation:
Department of Biomathematics, Institute of Physiology, The Czech Academy of Sciences, Videnska 1083, Prague 4, 14220, Czech Republic Department of Anatomy and Biomechanics, Faculty of Physical Education and Sport, Charles University, Jose Martiho 31, Prague 6, 162 00, Czech Republic Department of Biophysics, Biomedical Research Institute, Hasselt University, Agoralaan building C, Diepenbeek, B-3590, Belgium
Jiri Janacek
Affiliation:
Department of Biomathematics, Institute of Physiology, The Czech Academy of Sciences, Videnska 1083, Prague 4, 14220, Czech Republic
Eva Filova
Affiliation:
Department of Tissue Engineering, Institute of Experimental Medicine, The Czech Academy of Sciences, Videnska 1083, Prague 4, 14220, Czech Republic
Frantisek Lopot
Affiliation:
Department of Anatomy and Biomechanics, Faculty of Physical Education and Sport, Charles University, Jose Martiho 31, Prague 6, 162 00, Czech Republic
Rik Paesen
Affiliation:
Department of Biophysics, Biomedical Research Institute, Hasselt University, Agoralaan building C, Diepenbeek, B-3590, Belgium
Ondrej Fanta
Affiliation:
Department of Anatomy and Biomechanics, Faculty of Physical Education and Sport, Charles University, Jose Martiho 31, Prague 6, 162 00, Czech Republic
Anneliese Jarman
Affiliation:
Department of Tissue Engineering & Biophotonics, King’s College London, Guy’s Campus, Great Maze Pond, London, SE1 9RT, UK
Alois Necas
Affiliation:
Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1/3, Brno, 612 42, Czech Republic
Marcel Ameloot
Affiliation:
Department of Biophysics, Biomedical Research Institute, Hasselt University, Agoralaan building C, Diepenbeek, B-3590, Belgium
Karel Jelen
Affiliation:
Department of Biomathematics, Institute of Physiology, The Czech Academy of Sciences, Videnska 1083, Prague 4, 14220, Czech Republic
*
*Corresponding author. daniel.hadraba@fgu.cas.cz
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Abstract

Fibrillar collagen in tendons and its natural development in rabbits are discussed in this paper. Achilles tendons from newborn (~7 days) to elderly (~38 months) rabbits were monitored in intact (ntendons=24) and microtome sectioned (ntendons=11) states with label-free second harmonic generation microscopy. After sectioning, the collagen fiber pattern was irregular for the younger animals and remained oriented parallel to the load axis of the tendon for the older animals. In contrast, the collagen fiber pattern in the intact samples followed the load axis for all the age groups. However, there was a significant difference in the tendon crimp pattern appearance between the age groups. The crimp amplitude (A) and wavelength (Λ) started at very low values (A=2.0±0.6 µm, Λ=19±4 µm) for the newborn animals. Both parameters increased for the sexually mature animals (>5 months old). When the animals were fully mature the amplitude decreased but the wavelength kept increasing. The results revealed that the microtome sectioning artifacts depend on the age of animals and that the collagen crimp pattern reflects the physical growth and development.

Keywords

collagenagingcrimpfiber orientationtendon
Type
Biological Science Applications
Information
Microscopy and Microanalysis , Volume 23 , Issue 5 , October 2017 , pp. 1040 - 1047
Copyright
© Microscopy Society of America 2017 

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