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Characterization of Healthy and Fluorotic Enamel by Atomic Force Microscopy

Published online by Cambridge University Press:  03 September 2010

Verónica Zavala-Alonso ,
Gabriel A. Martínez-Castanon ,
Nuria Patiño-Marín ,
Humberto Terrones ,
Kenneth Anusavice  and
Juan P. Loyola-Rodríguez
Verónica Zavala-Alonso
Affiliation:
Advanced General Dentistry Program, San Luis Potosí University, México
Gabriel A. Martínez-Castanon
Affiliation:
Advanced General Dentistry Program, San Luis Potosí University, México
Nuria Patiño-Marín
Affiliation:
Advanced General Dentistry Program, San Luis Potosí University, México
Humberto Terrones
Affiliation:
Laboratory for Nanoscience, Nanotechnology and Advanced Materials Department, IPICYT, San Luis Potosi, SLP, Mexico
Kenneth Anusavice
Affiliation:
Dental Biomaterials, College of Dentistry, University of Florida, Gainesville, FL 32610, USA
Juan P. Loyola-Rodríguez*
Affiliation:
Advanced General Dentistry Program, San Luis Potosí University, México
*
Corresponding author. E-mail: jloyola@uaslp.mx
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Abstract

The aim was to characterize the external structure, roughness, and absolute depth profile (ADP) of fluorotic enamel compared with healthy enamel. Eighty extracted human molars were classified into four groups [TFI: 0, control (C); 1–3, mild (MI); 4–5, moderate (MO); 6–9, severe fluorosis (S)] according to the Thylstrup-Fejerskov Index (TFI). All samples were analyzed by atomic force microscopy.

The mean values of enamel surface roughness (ESR) in nm were: Group C, 92.6; Group MI, 188.8; Group MO, 246.9; and Group S, 532.2. The mean values of absolute depth profile in nm were: C, 1,065.7; MI, 2,360.7; MO, 2,536.7; and S, 6,146.2. The differences between mean ESR and mean ADP among groups were statistically significant (p < 0.05). This structural study confirms at the nanometer level that there is a positive association between fluorosis severity, ESR, and ADP, and there is an association with the clinical findings of fluorosis measured by TFI as well.

Keywords

healthy enamelfluorotic enamelAFM
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 16 , Issue 5 , October 2010 , pp. 531 - 536
Copyright
Copyright © Microscopy Society of America 2010

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