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CryoET of Single Particle CryoEM Grids Reveals Widespread Particle Adsorption to the Air-Water Interface, Which May be Reduced with New Plunging Techniques

Published online by Cambridge University Press:  01 August 2018

Alex J. Noble ,
Venkata P. Dandey ,
Hui Wei ,
Julia Brasch ,
Jillian Chase ,
Priyamvada Acharya ,
Yong Zi Tan ,
Zhening Zhang ,
Laura Y. Kim  and
Giovanna Scapin
...Show all authors
Alex J. Noble
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, NY, USA
Venkata P. Dandey
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, NY, USA
Hui Wei
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, NY, USA
Julia Brasch
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, NY, USA Department of Biochemistry and Molecular Biophysics, Columbia University, NYUSA
Jillian Chase
Affiliation:
Department of Chemistry and Biochemistry, City College of New York, NYUSA Ph.D. Programs in Biochemistry, The Graduate Center of the City University of New York, NYUSA
Priyamvada Acharya
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, NY, USA Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, MDUSA
Yong Zi Tan
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, NY, USA Department of Biochemistry and Molecular Biophysics, Columbia University, NYUSA
Zhening Zhang
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, NY, USA
Laura Y. Kim
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, NY, USA
Giovanna Scapin
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, NY, USA Department of Structural Chemistry and Chemical Biotechnology, Merck & Co., Inc., NYUSA
Micah Rapp
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, NY, USA Department of Biochemistry and Molecular Biophysics, Columbia University, NYUSA
Edward T. Eng
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, NY, USA
William J. Rice
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, NY, USA
Anchi Cheng
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, NY, USA
Carl J. Negro
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, NY, USA
Lawrence Shapiro
Affiliation:
Department of Biochemistry and Molecular Biophysics, Columbia University, NYUSA
Kwong
Affiliation:
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, MDUSA
David Jeruzalmi
Affiliation:
Department of Chemistry and Biochemistry, City College of New York, NYUSA Ph.D. Programs in Biochemistry, The Graduate Center of the City University of New York, NYUSA Ph.D. Programs in Biology, The Graduate Center of the City University of New York, NYUSA Ph.D. Programs in Chemistry, The Graduate Center of the City University of New York, NYUSA
Amedee des Georges
Affiliation:
Department of Chemistry and Biochemistry, City College of New York, NYUSA Ph.D. Programs in Biochemistry, The Graduate Center of the City University of New York, NYUSA Ph.D. Programs in Chemistry, The Graduate Center of the City University of New York, NYUSA Advanced Science Research Center at the Graduate Center of the City University of New York, NYUSA
Clinton S. Potter
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, NY, USA Department of Biochemistry and Molecular Biophysics, Columbia University, NYUSA
Bridget Carragher
Affiliation:
National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, NY, USA Department of Biochemistry and Molecular Biophysics, Columbia University, NYUSA

Abstract

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Type
Abstract
Information
Microscopy and Microanalysis , Volume 24 , Supplement S1: Proceedings of Microscopy & Microanalysis 2018 , August 2018 , pp. 872 - 873
Copyright
© Microscopy Society of America 2018 

References

[1] Noble, A, et al BioRxiv (2017).Google Scholar
[2] Glaeser, R Current Opinion in Colloid & Interface Science (2017) ..Google Scholar
[3] Martin, TG, et al, PNAS 113 2016) p. 7456.Google Scholar
[4] This work was supported by grants from the Simons Foundation (SF349247), NIH (GM103310, OD019994-01, R01-MH1148175, R01 GM084162, VRC intramural funding), NIMHD (5G12MD007603-30), A*STAR and the Agouron Institute [F00316].Google Scholar