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Deep Tissue Fluorescent Imaging in Scattering Specimens Using Confocal Microscopy

Published online by Cambridge University Press:  24 June 2011

Sherry G. Clendenon ,
Pamela A. Young ,
Michael Ferkowicz ,
Carrie Phillips  and
Kenneth W. Dunn
Sherry G. Clendenon*
Affiliation:
Department of Medicine, Division of Nephrology, Indiana University Medical Center, Indianapolis, IN 46202, USA
Pamela A. Young
Affiliation:
Department of Medicine, Division of Nephrology, Indiana University Medical Center, Indianapolis, IN 46202, USA
Michael Ferkowicz
Affiliation:
Herman B. Wells Center for Pediatric Research and Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN 46202, USA
Carrie Phillips
Affiliation:
Division of Nephrology and Department of Pathology, Indiana University School of Medicine, Indianapolis, IN 46202, USA
Kenneth W. Dunn
Affiliation:
Department of Medicine, Division of Nephrology, Indiana University Medical Center, Indianapolis, IN 46202, USA
*
Corresponding author. E-mail: sgclende@indiana.edu
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Abstract

In scattering specimens, multiphoton excitation and nondescanned detection improve imaging depth by a factor of 2 or more over confocal microscopy; however, imaging depth is still limited by scattering. We applied the concept of clearing to deep tissue imaging of highly scattering specimens. Clearing is a remarkably effective approach to improving image quality at depth using either confocal or multiphoton microscopy. Tissue clearing appears to eliminate the need for multiphoton excitation for deep tissue imaging.

Keywords

confocalmultiphotonclearingrefractive index matchingscattering
Type
Technology and Software Development Light and Confocal Microscopy
Information
Microscopy and Microanalysis , Volume 17 , Issue 4 , August 2011 , pp. 614 - 617
Copyright
Copyright © Microscopy Society of America 2011

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