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Effects of Mesua ferrea Leaf and Fruit Extracts on Growth and Morphology of Staphylococcus aureus

Published online by Cambridge University Press:  18 January 2013

Claira Arul Aruldass ,
Mani Maran Marimuthu ,
Surash Ramanathan ,
Sharif Mahsufi Mansor  and
Vikneswaran Murugaiyah
Claira Arul Aruldass
Affiliation:
School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia
Mani Maran Marimuthu
Affiliation:
Centre for Drug Research, Universiti Sains Malaysia, Penang, Malaysia
Surash Ramanathan
Affiliation:
Centre for Drug Research, Universiti Sains Malaysia, Penang, Malaysia
Sharif Mahsufi Mansor
Affiliation:
Centre for Drug Research, Universiti Sains Malaysia, Penang, Malaysia
Vikneswaran Murugaiyah*
Affiliation:
School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia
*
*Corresponding author. E-mail: vicky@usm.my
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Abstract

Mesua ferrea is traditionally used for treating bleeding piles, fever, and renal diseases. It has been reported to have antimircobial activity. In the present study, antibacterial efficacy of leaf and fruit extracts on the growth and morphology of Staphylococcus aureus is evaluated. Both extracts display good antibacterial activity against S. aureus with a minimum inhibition concentration of 0.048 mg/mL. Both extracts are bacteriostatic at a minimum bacteriostatic concentration of 0.39 mg/mL. The bacteriostatic activity lasts for 24 h, and then cells start to grow as normal as shown in time-kill analysis. Scanning electron microscopy study indicated potential detrimental effect of the extracts of leaf and fruits of M. ferrea on the morphology of S. aureus. The treatment with the extracts caused extensive lysis of the cells, leakage of intracellular constituents, and aggregation of cytoplasmic contents forming an open meshwork of the matrix.

Keywords

Staphylococcus aureusMesua ferreatime-kill analysisbacteriostaticscanning electron microscopymorphology
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 19 , Issue 1 , February 2013 , pp. 254 - 260
Copyright
Copyright © Microscopy Society of America 2013

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References

Abbas Ali, M., Abu Sayeed, M., Bhuiyan, M.S.A., Sohel, F.I. & Sarmina Yeasmin, M. (2004). Antimicrobial screening of Cassia fistula and Mesua ferrea . J Med Sci 4, 24–29.Google Scholar
Alam, M.S., Jain, N., Kamil, M. & Ilyas, M. (1987). Mesuein: A novel flavanone glycoside from Mesua ferrea . Chem Ind London 16, 565–566.Google Scholar
Archer, G.L. (1998). Staphylococcus aureus: A well-armed pathogen. Clin Inf Dis 26, 1179–1180.Google Scholar
Bala, K.R. & Seshadri, T.R. (1971). Isolation and synthesis of some coumarin components of Mesua ferrea seed oil. Phytochem 10, 1131–1134.Google Scholar
Banerji, R. & Chowdhury, A.R. (1993). Mesua ferrea: Chemical constituents and biological activity. J Chem Soc Pak 15(3), 207–211.Google Scholar
Chakraborty, D.P. & Bose, P. (1960). On the constitution of mesuol the bitter antibiotic principle of Mesua ferea linn. Part 1. Proc Natl Inst Sci India 26, 1–11.Google Scholar
Chakraborty, D.P. & Chatterji, D. (1969). Structure of mesuagin. A new 4-phenylcoumarin. J Org Chem 34, 3784–3786.Google Scholar
Chakraborty, D.P. & Das, B.C. (1966). The structure of mesuol. Tetrahedron Lett 46, 5727–5730.Google Scholar
Chakraborty, D.P., Purkayastha, M. & Bose, P.K. (1959). On antibiotic properties of some constituents of Mesua ferrea linn. Proc Natl Inst Sci India B Bio Sci 25, 8–11.Google Scholar
Claeson, P., Radstrom, P., Skold, O., Nilsson, A. & Hoglund, S. (1992). Bactericidal effect of the sesquiterpene t-cadinol on Staphylococcus aureus . Phytother Res 6, 94–98.CrossRefGoogle Scholar
Cox, S.D., Gustafson, J.E., Mann, C.M., Markham, J.L., Liew, Y.C., Hartland, R.P., Bell, H.C., Warmington, J.R. & Wyllie, S.G. (1998). Tea tree oil causes K+ leakage and inhibits respiration in Escherichia coli . Lett Appl Microbiol 26, 355–358.Google Scholar
de Billerbeck, V.G., Roques, C.G., Bessiere, J.M., Fonvieille, J.L. & Dargent, R. (2001). Effects of Cymbopogon nardus (L.) W. Watson essential oil on the growth and morphogenesis of Aspergillus niger . Can J Microbiol 47, 9–17.CrossRefGoogle Scholar
Dennis, T.J. & Akshaya Kumar, K. (1998). Constituents of Mesua ferrea . Fitoterapia 69, 291–304.Google Scholar
Dutta, P., Deb, N.C. & Bose, P.K. (1940). A preliminary note on mesuol, the bitter principle of Mesua ferrea . J Indian Chem Soc 17, 277–279.Google Scholar
Eloff, J.N. (1998). A sensitive and quick microplate method to determine the minimum inhibitory concentration of plant extracts for bacteria. Planta Med 64, 711–713.Google Scholar
Haznedaroglu, B.Z., Beyazit, Y., Walker, S.L. & Haznedaroglu, I.C. (2012). Pleiotropic cellular, hemostatic, and biological actions of Ankaferd hemostat. Crit Rev Onco/Hemato 83, 21–34.Google Scholar
Helander, I.M., Alakomi, H.L., Latva-Kala, K., Mattila-Sandholm, T., Pol, I., Smid, E.J., Gorris, L.G. & Von Wright, A. (1998). Characterization of the action of selected essential oil components on gram-negative bacteria. J Agric Food Chem 46, 3590–3595.Google Scholar
Holden, M.T.G., Feil, E.J., Lindsay, J.A., Peacock, S.J., Day, N.P.J., Enright, M.C., Foster, T.J., Moore, C.E., Hurst, L., Atkin, R., Barron, A., Bason, N., Bentley, S.D., Chillingworth, T., Churcher, C., Clark, L., Corton, C., Cronin, A., Dogget, J., Dowd, L., Feltwell, T., Hance, Z., Harris, B., Hauser, H., Holroyd, S., Jagels, K., James, K.D., Lennard, N., Line, A., Mayes, R., Moule, S., Mungall, K., Ormond, D., Quail, M.A., Rabbinowitsch, E., Rutherford, K., Mandy, S.K.M., Sharp, S., Simmonds, M., Kim, S.K., Whitehead, S., Bart, G., Barrell, B.G., Brian, G., Spratt, B.G. & Parkhill, J. (2004). Complete genomes of two clinical Staphylococcus aureus strains: Evidence for the rapid evolution of virulence and drug resistence. PNAS 101(26), 9786–9791.Google Scholar
Iwalokum, B.A., Ogunlendun, A., Ogbolu, D.O., Bamiro, S.B. & Jimi-Omojola, J. (2004). In vitro antimicrobial properties of aqueous garlic extract against multidrug-resistant bacteria and Candida species from Nigeria. J Med Food 7(3), 327–333.Google Scholar
Karou, D., Dicko, M.H., Simpore, J. & Traore, A.S. (2005). Antioxidant and antibacterial activities of polyphenols form ethnomedicinal plants of Burkina Faso. Afr J Biotechnol 4(8), 823–828.Google Scholar
Kim, J.M., Marshall, M.R., Cornell, J.A., Preston, J.F. & Wei, C.I. (1995). Antibacterial activity of carvacrol, citral, and geraniol against Salmonella typhimurium in culture medium and on fish cubes. J Food Sci 60, 1364–1368.Google Scholar
Knobloch, K., Pauli, A., Iberl, B., Weigand, H. & Weis, N. (1989). Antibacterial and antifungal properties of essential oil components. J Essent Oil Res 1, 119–128.Google Scholar
Luisella, V., Erminio, L., Giovanni, V., Paola, V. F., Maria, G. N., Alessandro, R., Silvia, P., Donatella, T., Antonella, R. & Ezio, B. (2004). 4-alkyl- and 4-phenylcoumarins from Mesua ferrea as promising multidrug resistant antibacterials. Phytochem 65, 2867–2879.Google Scholar
McDowell, E.M. & Trump, B.F. (1976). Histologic fixatives suitable for diagnostic light and electron microscopy. Arch Pathol Lab Med 100, 405–414.Google Scholar
Mohammad, A.B.R., Zakaria, Z., Sreenivasan, S., Lachimanan, Y.L. & Santhanam, A. (2010). Assessment of Euphorbia hitta L. leaf, flower, stem and root extracts for their antibacterial and antifungal activity and brine shrimp lethality. Molecules 15, 6008–6018.Google Scholar
Mylotte, J.M., McDermott, C. & Spooner, J.A. (1987). Prospective study of 114 constitutive episodes of staphylococcus aureus bacteremis. Rev Infectious Diseases 9, 891–907.Google Scholar
Pardo de Tavera, T.H. (2008). The medicinal plants of the Philippines. In The Project Gutenberg EBook of the Medicinal Plants of Philippines, Beers, J. (trans.), p. 40. Philadelphia, PA: Thomas, P. Blakiston's Son and Co. Google Scholar
Parshuram, M., Pankaj, A., Rajeev, R., Kameshwar, S. & Sandeep, G. (2009). In vivo antioxidant of methanolic extract of Mesua ferrea linn. Int J Pharm Tech Res 1(4), 1692–1696.Google Scholar
Perry, L.M. & Metzger, J. (1980). Medicinal Plants of East and Southeast Asia. Cambridge, MA: MIT Press.Google Scholar
Sanders, C.C. & Sanders, W.E. Jr. (1992). β-Lactamase resistance in gram-negative bacteria: Global trends and clinical impact. Clin Infec Dis 15, 824–839.Google Scholar
Sangetha, S., Sasidharan, S., Zuraini, Z. & Suryani, S. (2008). Fungicidal effect and oral acute toxicity of Cassia spectabilis leaf extract. Jpn J Med Mycol 49, 299–304.Google Scholar
Shan, B., Cai, Y.Z., Brooks, J.D. & Corke, H. (2007). Antibacterial properties and major bioactive components of cinnamon stick (Cinnamomum burmannii): Activity against foodborne pathogenic bacteria. J Agric Food Chem 55, 5484–5490.Google Scholar
Shin, I.S, Hideki, M. & Kinae, N. (2004). Bacterial activity of wasabi (Wasabia japonica) against Helicobacter pylori . Int J Food Microbiol 94, 255–261.Google Scholar
Shome, U., Mehrotra, S. & Sharma, H.P. (1982). Pharmacognostic studies on the flower of Mesua ferrea L. Proc Indian Acad Sci 9, 211–226.Google Scholar
Sibanda, T., Olaniran, A.O. & Okoh, A.I. (2010). In vitro antibacterial activities of crude extracts of Garcinia kola seeds against wound sepsis associated Stapylococcus strains. J Med Plants Res 4(8), 710–716.Google Scholar
Tasdelen Fisgin, N., Cayci, Y.T., Coban, A.Y., Ozalti, D., Tanyel, E., Durupinar, B. & Tulek, N. (2009). Antimicrobial activity of plant extract Ankaferd Blood Stopper. Fitoterapia 80, 48–50.Google Scholar
Tenover, F.C., Weigel, L.M., Appelbaum, P.C., McDougal, L.K., Chaitram, J., McAlister, S., Clark, N., Killgore, G., O'Hara, C.M., Jevitt, L., Patel, J.B. & Bozdogan, B. (2004). Vancomycin-resistant Staphylococcus aureus isolate from a patient in Pennsylvania. Antimicrob Agents Ch 48(1), 275–280.Google Scholar
Ultee, A., Bennik, M.H.J. & Moezelaar, R. (2002). The phenolic hydroxyl group of carvacrol is essential for action against the food-borne pathogen Bacillus cereus . Appl Environ Microbiol 68, 1561–1568.Google Scholar
Weber, D.J., Hoffmann, K.K., Rutala, W.A. & Pyatt, D.G. (2009). Control of healthcare-associated Staphylococcus aureus: Survey of practices in North Carolina hospitals. Infect Control Hosp Epidemiol 30, 909–911.Google Scholar
Zhu, S.Y., Yang, Y., Yu, H.D., Ying, Y. & Zou, G.L. (2005). Chemical composition and antimicrobial activity of essential oils of Chrysanthemum indicum . J Ethnopharmacol 96, 151–158.Google Scholar