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Efficient, Retro Viral-Mediated Expression Of GFP Fusion Proteins as Inhibitors of Intracellular Signal Transduction Pathways: A Case Study

Published online by Cambridge University Press:  02 July 2020

T.J. Murphy  and
Xiaofei Wang
T.J. Murphy
Affiliation:
Department of Pharmacology, and the Graduate Program in Molecular Therapeutics and Toxicology, Graduate Division of Biological and Biomedical Sciences, Emory University School of Medicine, Atlanta, GA30322
Xiaofei Wang
Affiliation:
Department of Pharmacology, and the Graduate Program in Molecular Therapeutics and Toxicology, Graduate Division of Biological and Biomedical Sciences, Emory University School of Medicine, Atlanta, GA30322
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Extract

A variety of extracellular signals, including hormones, neurotransmitters, growth factors and cytokines, are capable of activating vascular smooth muscle cells (VSMC). These can modulate the VSMC phenotype, which underlies the progression of vascular diseases. We study gene expression control responses in VSMC that are regulated by different classes of cell surface receptors. Taken as simpler model systems for the pleiotropic sensitivity of VSMC to extracellular stimuli, we focus on understanding the elements of intracellular signal transduction pathways involved in controlling gene expression. In particular, we are interested in identifying signaling elements that can be activated by multiple classes of cell surface receptors.

Previous studies had supported both a role of protein kinase A (PKA) activity and transcriptional induction of an unknown factor in control of ATI angiotensin receptor gene expression in VSMC (1). The mRNA from this gene is down-regulated by a variety of extracellular stimuli, and we hypothesized that stimulation of PKA by several classes of receptors might explain the apparently pleiotropic nature of ATI-receptor down regulation.

Type
Detection and Application of Green (and other Colored) Fluorescent Proteins
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1012 - 1013
Copyright
Copyright © Microscopy Society of America

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References

1.Wang, X.-F., Nickenig, G., and Murphy, T. J. (1997). The vascular smooth muscle type I angiotensin II receptor mRNA is destabilized by cAMP-elevating agents. Mol. Pharmacol. 52, 781–787.CrossRefGoogle Scholar
2.Walsh, D. A., Angelos, K. L., Van Patten, S. M., Glass, D. B., and Garetto, L. P. (1990). The inhibitor of the cAMP-dependent protein kinase.In Peptides and Protein Phosphorylation, Kemp, B. E., ed. (Boca Raton, CRC Press, Inc), pp. 43–84.Google Scholar
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