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Electron Holography Study of the Charging Effect in Microfibrils of Sciatic Nerve Tissues

Published online by Cambridge University Press:  06 August 2013

Ki Hyun Kim ,
Zentaro Akase ,
Daisuke Shindo ,
Nobuhiko Ohno ,
Yasuhisa Fujii ,
Nobuo Terada  and
Shinichi Ohno
Ki Hyun Kim
Affiliation:
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai 980-8577, Japan
Zentaro Akase
Affiliation:
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai 980-8577, Japan
Daisuke Shindo*
Affiliation:
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai 980-8577, Japan
Nobuhiko Ohno
Affiliation:
Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi 409-3898, Japan
Yasuhisa Fujii
Affiliation:
Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi 409-3898, Japan
Nobuo Terada
Affiliation:
Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi 409-3898, Japan
Shinichi Ohno
Affiliation:
Department of Anatomy and Molecular Histology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi 409-3898, Japan
*
*Corresponding author. E-mail: shindo@tagen.tohoku.ac.jp
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Abstract

The charging effects of microfibrils of sciatic nerve tissues due to electron irradiation are investigated using electron holography. The phenomenon that the charging effects are enhanced with an increase of electron intensity is visualized through direct observations of the electric potential distribution around the specimen. The electric potential at the surface of the specimen could be quantitatively evaluated by simulation, which takes into account the reference wave modulation due to the long-range electric field.

Keywords

electron holographysecondary electronselectric potential distributioncharging effectmicrofibrilphase reconstructionamplitude reconstruction
Type
Research Article
Information
Microscopy and Microanalysis , Volume 19 , Supplement S5: IUMAS , August 2013 , pp. 54 - 57
Copyright
Copyright © Microscopy Society of America 2013 

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Footnotes

Primary institution where the research was performed is the Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Japan

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