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Extraction of Three-Dimensional Information of Biological Membranous Tissue with Scanning Confocal Infrared Laser Microscope Tomography

Published online by Cambridge University Press:  06 August 2013

Soonwook Kwon ,
Se-Bum Choi ,
Min Gyu Park ,
Hyunung Yu ,
Seung-Woo Suh  and
Im Joo Rhyu
Soonwook Kwon
Affiliation:
Department of Anatomy, Korea University College of Medicine, Seoul 136-705, Korea
Se-Bum Choi
Affiliation:
Korea Research Institute of Standards and Science, Daejon 305-340, Korea
Min Gyu Park
Affiliation:
Korea Research Institute of Standards and Science, Daejon 305-340, Korea
Hyunung Yu*
Affiliation:
Korea Research Institute of Standards and Science, Daejon 305-340, Korea
Seung-Woo Suh
Affiliation:
Department of Orthopedics, Korea University Guro Hospital, Seoul 152-703, Korea
Im Joo Rhyu*
Affiliation:
Department of Anatomy, Korea University College of Medicine, Seoul 136-705, Korea
*
†Corresponding author. E-mail: peacewithu@kriss.re.kr
‡Corresponding author. E-mail: irhyu@korea.ac.kr
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Abstract

The “LEXT” confocal laser scanning microscope has been used for the three-dimensional (3D) imaging of the surface of specimens, especially in materials science fields, by the penetration of near-infrared (NIR) light without mechanical cutting, deposition, or other specimen pretreatment. Noninvasive investigation of various biological tissues such as human spinal dura mater, rat aorta, and cornea without the dehydration process was successfully carried out with the “LEXT,” in order to access both surface and internal topographic images of the biological structures at a good status of the wet tissue such as in vivo, especially in measuring tissue thickness. The confocal NIR laser microscopy offers the viable means to visualize tissue architecture and its thickness in microdomain to integrate 3D images efficiently. We believe that the “LEXT” has a good application for biological researchers to study biomaterials, and it would be useful as a diagnostic tool in the near future.

Keywords

laser microscopeimagingconfocalinfraredscanningdura matermembrane
Type
Research Article
Information
Microscopy and Microanalysis , Volume 19 , Supplement S5: IUMAS , August 2013 , pp. 194 - 197
Copyright
Copyright © Microscopy Society of America 2013 

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