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FEISEM, Form and Function in the Nuclear Pore Complex

Published online by Cambridge University Press:  02 July 2020

T.D. Allen ,
G. R. Bcnnion ,
S. A. Rutherford ,
E. Kiscleva  and
M. W. Goldberg
T.D. Allen
Affiliation:
Section of Cell & Tumour Biology, Paterson Institute for Cancer Research, Christie Hospital, Wilmslovv Road, M20 9NX
G. R. Bcnnion
Affiliation:
Section of Cell & Tumour Biology, Paterson Institute for Cancer Research, Christie Hospital, Wilmslovv Road, M20 9NX
S. A. Rutherford
Affiliation:
Section of Cell & Tumour Biology, Paterson Institute for Cancer Research, Christie Hospital, Wilmslovv Road, M20 9NX
E. Kiscleva
Affiliation:
Institute ofCytology & Genetics, Novosibirsk 90, Russia630090
M. W. Goldberg
Affiliation:
Section of Cell & Tumour Biology, Paterson Institute for Cancer Research, Christie Hospital, Wilmslovv Road, M20 9NX
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Extract

Recent initiatives have resulted in a considerable increase in our understanding of the structure of the nuclear pore complex (NPC). The biochemical factors involved in both import and export have been rapidly characterised, with steady progress in the molecular dissection of the structural elements of the NPC, which is a unit of considerable molecular architecture (MW 125 kD), comprising an estimated 50- 100 different proteins. Despite this progress, the crucial molecular interactions involved in the mechanics of transport through the central transporter of the NPC remain unclear. NPC structure in Diptera, fish, (Fig 1) amphibians, birds and mammals shows a high degree of evolutionary conservation. 3D reconstructions of isolated yeast NPCs, show that the core structure is very similar to ‘higher’ organisms, but peripheral structures may be considerably reduced in structural complexity (1).

Individual NPC components have been accessed in FEISEM by a variety of methods, including proteolysis,

Type
Chambers and Channels: Functional Connections in Multiprotein Complexes Studied by Single Chambers and Channels
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 958 - 959
Copyright
Copyright © Microscopy Society of America

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References

References:

1.Yang, Q., M P., Rout, Akey, C W., Mol Cell 1998 1 223–234CrossRefGoogle Scholar
2.Allen, T D., Bennion, G R., Rutherford, S A., Reipert, S., Ramalho, A., Goldberg, M W., Scanning 1997 19 403–410CrossRefGoogle ScholarPubMed
3.Goldberg, M W., Weise, C, Allen, T.D., Wilson, K., J Cell Sci 1997 110 409–420CrossRefGoogle Scholar
4.Kiseleva, E., Goldberg, M W., Daneholt, B., Allen, T.D.,J Mol Biol 1996 260 304–311CrossRefGoogle Scholar