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Focused X-Ray Histological Analyses to Reveal Asbestos Fibers and Bodies in Lungs and Pleura of Asbestos-Exposed Subjects

Published online by Cambridge University Press:  13 September 2016

Lorella Pascolo ,
Alessandra Gianoncelli ,
Clara Rizzardi ,
Martin de Jonge ,
Daryl Howard ,
David Paterson ,
Francesca Cammisuli ,
Murielle Salomé ,
Paolo De Paoli  and
Mauro Melato
...Show all authors
Lorella Pascolo*
Affiliation:
Department of Medical, Surgical, and Health Sciences, University of Trieste, 34149 Trieste, Italy
Alessandra Gianoncelli
Affiliation:
Elettra—Sincrotrone Trieste, Basovizza, 34149 Trieste, Italy
Clara Rizzardi
Affiliation:
Department of Medical, Surgical, and Health Sciences, University of Trieste, 34149 Trieste, Italy
Martin de Jonge
Affiliation:
Australian Synchrotron, 3168 Clayton, Melbourne, VIC, Australia
Daryl Howard
Affiliation:
Australian Synchrotron, 3168 Clayton, Melbourne, VIC, Australia
David Paterson
Affiliation:
Australian Synchrotron, 3168 Clayton, Melbourne, VIC, Australia
Francesca Cammisuli
Affiliation:
Department of Medical, Surgical, and Health Sciences, University of Trieste, 34149 Trieste, Italy
Murielle Salomé
Affiliation:
European Synchrotron Radiation Facility, 38000 Grenoble, Cedex 9, France
Paolo De Paoli
Affiliation:
Scientific Direction, Centro di Riferimento Oncologico (CRO), National Cancer Institute, IRCCS, 33081 Aviano (PN), Italy
Mauro Melato
Affiliation:
Institute for Maternal and Child Health, IRCCS Burlo Garofolo, 34137 Trieste, Italy
Vincenzo Canzonieri
Affiliation:
Division of Pathology, Centro di Riferimento Oncologico (CRO), National Cancer Institute, IRCCS, 33081 Aviano (PN), Italy
*
*Corresponding author.lorella.passcolo@gmail.com
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Abstract

Asbestos bodies are the histological hallmarks of asbestos exposure. Both conventional and advanced techniques are used to evaluate abundance and composition in histological samples. We previously reported the possibility of using synchrotron X-ray fluorescence microscopy (XFM) for analyzing the chemical composition of asbestos bodies directly in lung tissue samples. Here we applied a high-performance synchrotron X-ray fluorescence (XRF) set-up that could allow new protocols for fast monitoring of the occurrence of asbestos bodies in large histological sections, improving investigation of the related chemical changes. A combination of synchrotron X-ray transmission and fluorescence microscopy techniques at different energies at three distinct synchrotrons was used to characterize asbestos in paraffinated lung tissues. The fast chemical imaging of the XFM beamline (Australian Synchrotron) demonstrates that asbestos bodies can be rapidly and efficiently identified as co-localization of high calcium and iron, the most abundant elements of these formations inside tissues (Fe up to 10% w/w; Ca up to 1%). By following iron presence, we were also able to hint at small asbestos fibers in pleural spaces. XRF at lower energy and at higher spatial resolution was afterwards performed to better define small fibers. These analyses may predispose for future protocols to be set with laboratory instruments.

Keywords

asbestosX-ray fluorescenceironcalciumlung
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 22 , Issue 5 , October 2016 , pp. 1062 - 1071
Copyright
© Microscopy Society of America 2016 

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