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Functional Imaging of Neurotransmitters in Hymenolepis diminuta Treated with Senna Plant Through Light and Confocal Microscopy

Part of: Micrographia Collection

Published online by Cambridge University Press:  13 November 2018

Bidisha Ukil ,
Suman Kundu  and
Larisha Mawkhlieng Lyndem
Bidisha Ukil
Affiliation:
Parasitology Research Laboratory, Department of Zoology, Siksha Bhavana, Visva-Bharati University, Santiniketan 731235, West Bengal, India
Suman Kundu
Affiliation:
Parasitology Research Laboratory, Department of Zoology, Siksha Bhavana, Visva-Bharati University, Santiniketan 731235, West Bengal, India
Larisha Mawkhlieng Lyndem*
Affiliation:
Parasitology Research Laboratory, Department of Zoology, Siksha Bhavana, Visva-Bharati University, Santiniketan 731235, West Bengal, India
*
Author for correspondence: Larisha Mawkhlieng Lyndem, E-mail: lyndemlarisha@gmail.com
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Abstract

Previous studies have shown the anthelmintic efficacy of Senna alata, Senna alexandrina and Senna occidentalis on the zoonotic parasite Hymenolepis diminuta through microscopic studies on morphological structure. The present study is based on the light and confocal microscopic studies to understand if Senna extracts affect neurotransmitter activity of the parasites. A standard concentration (40 mg/mL) of the three leaf extracts and one set of 0.005 mg/mL concentration of the reference drug praziquantel were tested against the parasites, keeping another set of parasites in phosphate buffer saline as a control. Histochemical studies were carried out using acetylthiocholine iodide as the substrate and acetylcholinesterase as the marker enzyme for studying the expression of the neurotransmitter of the parasite and the staining intensity was observed under a light microscope. Immunohistochemical studies were carried out using anti serotonin primary antibody and fluorescence tagged secondary antibody and observed using confocal microscopy. Intensity of the stain decreases in treated parasites compared with the control which implies loss of activity of the neurotransmitters. These observations indicated that Senna have a strong anthelmintic effect on the parasite model and thus pose as a potential anthelmintic therapy.

Keywords

anthelminticparasiteacetylcholinesteraseserotoninSenna
Type
Micrographia
Information
Microscopy and Microanalysis , Volume 24 , Issue 6 , December 2018 , pp. 734 - 743
Copyright
© Microscopy Society of America 2018 

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Footnotes

Cite this article: Ukil B, Kundu S and Lyndem LM (2018) Functional Imaging of Neurotransmitters in Hymenolepis diminuta Treated with Senna Plant Through Light and Confocal Microscopy. Microsc Microanal. 24(6), 734–743. doi: 10.1017/S143192761801526X

References

Aubry, ML, Cowell, P, Davey, MG Shevde, S (1980) Aspects of the pharmacology of a new anthelmintic: Pyrantel. Br J Pharmacol 38, 332–344.Google Scholar
Barker, LR, Bueding, E Timms, AR (1966) The possible role of acetylcholine in Schistosoma mansoni . Br J Pharmacol 26, 656–665.Google Scholar
Bennett, J Bueding, E (1971) Localization of biogenic amines in Schistosoma mansoni . Comp Biochem Physiol 39, 859–867.Google Scholar
Bueding, E (1952) Acetylcholinesterase activity of Schistosoma mansoni . Br J Pharmacol 7, 563– 566.Google Scholar
Bueding, E, Schiller, EL Bourgeouis, JG (1966) Some physiological, biochemical and morphologic effects of tris(p-aminophenyl) carbonium salts (TAC) on S. mansoni . Am J Trop Med Hyg 16, 500–515.Google Scholar
Chance, MRA Mansour, TE (1953) A contribution to the pharmacology of movement in the liver fluke. Br J Pharmacol Chemother 8, 134–138.Google Scholar
Coles, GC, East, GM Jenkins, SN (1975) The mechanism of action of the anthelmintic levamisole. Gen Pharmacol 6, 309–313.Google Scholar
Ĉolović, MB, Krstić, DZ, Lazarević-Pašti, TD, Bondžić, AM Vasić, VM (2013) Acetylcholinesterase inhibitors: Pharmacology and toxicology. Curr Neuropharmacol 11, 315–335.Google Scholar
Cox, FEG (1994) Immunology. In Modern Parasitology, Cox FEG (Ed.), pp. 193–218. Oxford: Blackwell Scientific Publications.Google Scholar
Crisford, A, Murray, C, O’Connor, V, Edwards, RJ, Kruger, N, Welz, C, von Samson-Himmelstjerna, G, Harder, A, Walker, RJ Holden-Dye, L (2011) Selective toxicity of the anthelmintic emodepside revealed by heterologous expression of human KCNMA1 in Caenorhabditis elegans . Mol Pharmacol 79(6), 1031–1043.Google Scholar
Day, TA Maule, AG (1999) Parasitic peptides! The structure and function of neuropeptides in parasitic worms. Peptides 20, 999–1019.Google Scholar
Ellman, GL, Courtney, KD, Andres, V Jr. Featherstone, RM (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7(2), 88–90.Google Scholar
Feldman, JM (2004) Encyclopedia of Gastroenterology. New York: Academic Press.Google Scholar
Geary, TG, Klein, RD, Vanover, L, Bowman, JW Thompson, DP (1992) The nervous systems of helminths as targets for drugs. J Parasitol 78(2), 215–230.Google Scholar
Gomori, G (1952) Histochemical demonstration of sites of choline esterase activity. Proc Soc Exp Biol Med 68, 354–358.Google Scholar
Gustafsson, MKS (1991) Skin the tapeworms before you stain their nervous system!. Parasitol Res 77(6), 509–516.Google Scholar
Halton, DW (2004) Microscopy and the helminth parasite. Micron 35, 361–390.Google Scholar
Harsha, MN, Dulloo, P, Rupachandra, S, Jagadeeshwari, S, Davina, JM Porkodi, S (2018) Isolation and identification of antimicrobial proteins from the leaves of Valeriana hardwickii and Senna obtusifolia . Asian J Pharm Clin Res 11, 438–440.Google Scholar
Hovis, DV Heuer, AH (2010) The use of laser scanning confocal microscopy (LSCM) in materials science. J Microsc 240(3), 173–180.Google Scholar
Hrčkova, G, Velenbný, S, Halton, DW Maule, AG (2002) Mesocestoides corti (syn. M. vogae): Modulation of larval motility by neuropeptides, serotonin and acetylcholine. Parasitology 124, 409–421.Google Scholar
Hu, Y, Xiao, SH Aroian, RV (2009) The new anthelmintic tribendimidine is an L type (levamisole and pyrantel) nicotinic acetylcholine receptor agonist. PLoS Negl Trop Dis 3, e499.Google Scholar
Johnston, CF, Shaw, C, Halton, DW Fairweather, I (1990) Confocal scanning laser microscopy and helminth neuroanatomy. Parasitol Today 6(9), 305–308.Google Scholar
Kaminsky, R, Ducray, P, Jung, M, Clover, R, Rufener, L, Bouvier, J, Schorderet Weber, S, Wenger, A, Wieland-Berghausen, S, Goebel, T, Gauvry, N, Pautrat, F, Skripsky, T, Froelich, O, Komoin-Oka, C, Westlund, B, Sluder, A Maser, P (2008) A new class of anthelmintics effective against drug-resistant nematodes. Nature 452, 176–180.Google Scholar
Karnovsky, MJ Roots, L (1964) A “direct-coloring” thiocholine method for cholinesterases. J Histochem Cytochem 12, 219–221.Google Scholar
Karthika, C, Mohammad, RK Manivannan, S (2016) Phytochemical analysis and evaluation of antimicrobial potential of Senna alata Linn. leaves extract. Asian J Pharm Clin Res 9, 253–257.Google Scholar
Kemmerling, U, Cabrera, G, Campos, EO, Inestrosa, NC Galanti, N (2006) Localization, specific activity, and molecular forms of acetylcholinesterase in developmental stages of the cestode Mesocestoides corti . J Cell Physiol 206(2), 503–509.Google Scholar
Khare, P, Kishore, K Sharma, DK (2017) A study on the standardization parameters of Cassia angustifolia . Asian J Pharm Clin Res 10, 329–332.Google Scholar
Kopp, SR, Kotze, AC, McCarthy, JS, Traub, RJ Coleman, GT (2008) Pyrantel in small animal medicine: 30 years on. Vet J 178, 177–184.Google Scholar
Kundu, S, Roy, S Lyndem, LM (2012) Cassia alata L.: Potential role as anthelmintic agent against Hymenolepis diminuta . Parasitol Res 111(3), 1187–1192.Google Scholar
Kundu, S, Roy, S, Nandi, S, Ukil, B Lyndem, LM (2015) In vitro anthelmintic effects of Senna occidentalis (L.) Link (Leguminosae) on rat tapeworm Hymenolepis diminuta. Int J Pharm Pharm Sci 7(6), 268–271.Google Scholar
Kundu, S, Roy, S, Nandi, S, Ukil, B Lyndem, LM (2016) Senna alexandrina Mill. induced ultrastructural changes in Hymenolepis diminuta . J Parasit Dis 41(1), 147–154.Google Scholar
Lee, MB, Bueding, E Schiller, EL (1978) The occurrence and distribution of 5 hydroxytryptamine in Hymenolepis diminuta and H. nana . J Parasitol 64, 257–264.Google Scholar
Leitch, B Probert, AJ (1984) Schistosoma haematobium: Amoscanate and adult worm ultrastructure. Exp Parasitol 58, 278–289.Google Scholar
Liu, Z, Williamson, MS, Landsell, SJ, Denhom, I, Han, Z Miller, NS (2005) A nicotinic acetylcholine receptor mutation conferring target-site resistance to imidacloprid in Nilaparvata lugens (brown planthopper). Proc Nat Acad Sci U S A 102, 8420–8425.Google Scholar
Martin, RJ (1985) Gamma-aminobutyric acid and piperazine activated single channel currents from Ascaris suum body muscle. Br J Pharmacol 84, 445–461.Google Scholar
Maule, AG, Halton, DW, Jhonston, CF, Shaw, C Fairweather, I (1990) The serotoninergic, cholinergic and peptidergic components of the nervous system in the monogenean parasite, Diclidophora merlangi: A cytochemical study. Parasitology 100, 255–273.Google Scholar
Maule, AG, Mousley, A, Marks, NJ, Day, TA, Thompson, DP, Geary, TG Halton, DW (2002) Neuropeptide signaling systems – Potential drug targets for parasite and pest control. Curr Top Med Chem 2, 733–758.Google Scholar
Molina, V, Ciesielski, L, Gobaille, S, Isel, F Mandel, P (1987) Inhibition of mouse killing behavior by serotonin-mimetic drugs: Effects of partial alterations of serotonin neurotransmission. Pharmacol Biochem Behav 27, 123–131.Google Scholar
Odeja, O, Obi, G, Ogwuch, CE, Elemike, EE Oderinlo, Y (2015) Phytochemical screening, antioxidant and antimicrobial activities of Senna occidentalis (L.) leaves extract. Clin Phytosci 1, 6.Google Scholar
Ott, P, Jerry, B Broadback, V (1975) Multiple molecular forms of purified human erythrocyte acetylcholinesterase. Environ J Biochem 57, 469–480.Google Scholar
Pal, P Tandon, V (1998) Anthelmintic efficacy of Flemingia vestita (Leguminoceae): Genistein induced alterations in the activity of tegumental enzymes in the cestode, Raillietina echinobothrida . Parasitol Int 47, 233–243.Google Scholar
Pax, RA, Siefker, C Bennet, JL (1984) Schistosoma mansoni: Differences in acetylcholine, dopamine and serotonin control of circular and longitudinal parasite muscle. Exp Parasitol 58, 314–324.Google Scholar
Pearse, AGE (1968) Histochemistry: Theoretical and Applied. New York: Churchill Livingstone.Google Scholar
Ramisz, A (1967) Studies on the nervous system of nematodes and cestodes by means of a histochemical method for active acetylcholinesterase. Acta Parasitol Pol 14, 365–380.Google Scholar
Ribeiro, P, El-Shehabiandn, F Patocka, N (2005) Classical transmitters and their receptors in flatworms. Parasitology 131, S19–S40.Google Scholar
Robertson, AP, Clark, CL, Burns, TA, Thompson, DP, Geary, TG, Trailovic, SM Martin, RJ (2002) Paraherquamide and 2-deoxy-paraherquamide distinguish cholinergic receptor subtypes in Ascaris muscle. J Pharmacol Exp Ther 302, 853–860.Google Scholar
Rozario, T Newmark, PA (2015) A confocal microscopy-based atlas of tissue architecture in the tapeworm Hymenolepis diminuta . Exp Parasitol 158, 31–41.Google Scholar
Sager, H, Hosking, B, Bapst, B, Stein, P, Vanhoff, K Kaminsky, R (2009) Efficacy of the amino-acetonitrile derivative, monepantel, against experimental and natural adult stage gastro-intestinal nematode infections in sheep. Vet Parasitol 159, 49–54.Google Scholar
Samii, SI Webb, RA (1990) Acetylcholine-like immunoreactivity in the cestode Hymenolepis diminuta . Brain Res 513, 161–165.Google Scholar
Sattelle, DB (2009) Invertebrate nicotinic acetylcholine receptors – Targets for chemicals and drugs important in agriculture, veterinary medicine and human health. J Pestic Sci 34, 233–240.Google Scholar
Sermakkani, M Thangapandian, V (2012) GC-MS analysis of Cassia italica leaf methanol extract. Asian J Pharm Clin Res 5, 90–94.Google Scholar
Srivastava, VK, Maheshwari, ML Mandal, S (1983) A rapid high performance liquid chromatography method for analysis of sennoside in Senna . Ind J Pharm Sci 45, 230–233.Google Scholar
Sukhdeo, MVK, Hsu, SC, Thompson, CS Mettrick, DF (1984) Hymenolepis diminuta: Behavioral effects of 5-hydroxytryptamine, acetylcholine, histamine and somatostatin. J Parasit 70, 682–688.Google Scholar
Sukhdeo, MVK, Sangster, NC Mettrick, DF (1986) Effects of cholinergic drugs on longitudinal muscle contractions of Fasciola hepatica . J Parasitol 72, 492–497.Google Scholar
Sundaraneedi, M, Eichenberger, RM, Al-Hallaf, R, Yang, D, Sotillo, J, Rajan, S, Wangchuk, P, Giacomin, PR, Keene, FR, Loukas, A, Collins, JG Pearson, MS (2018) Polypyridylruthenium(II) complexes exert in vitro and in vivo nematocidal activity and show significant inhibition of parasite acetylcholinesterases. Int J Parasitol Drugs Drug Resist 8, 1–7.Google Scholar
Thompson, CS, Sangster, NC Mettrick, DF (1986) Cholinergic inhibition of muscle contraction in Hymenolepis diminuta (Cestoda). Can J Zool 64, 2111–2115.Google Scholar
Tin, W, Hla, P Khin, KS (1994) Anticholinesterase activities of some anthelmintic agents and some medicinal plants. Myanmar Health Sci Res J 6, 70–74.Google Scholar
Viswanathan, S Nallamuthu, T (2012) Phytochemical screening and antimicrobial activity of leaf extracts of Senna alexandrina Mill. against human pathogens. Int J Curr Sci 2, 51–56.Google Scholar
Voge, M Bueding, E (1980) Schistosoma mansoni: Tegumental structure alterations induced by subcurative doses of the schistosomicide amoscanate. Exp Parasitol 50, 251–259.Google Scholar
Wilson, VCLC Schiller, EL (1969) The neuroanatomy of Hymenolepis diminuta and Himenolepis nana . J Parasitol 55, 261–270.Google Scholar
Yadav, VK (2013) Serotonin: The central link between bone mass and energy metabolism. In Translational Endocrinology of Bone, Karnesty G (Ed.), pp. 51–62. New York: Elsevier.Google Scholar