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Gray-Level Co-Occurrence Matrix Analysis of Granule Neurons of the Hippocampal Dentate Gyrus Following Cortical Injury

Published online by Cambridge University Press:  17 January 2020

Igor Pantic ,
Rada Jeremic ,
Sanja Dacic ,
Sanja Pekovic ,
Senka Pantic ,
Marina Djelic ,
Zagorka Vitic ,
Predrag Brkic  and
Claude Brodski
Igor Pantic
Affiliation:
Faculty of Medicine, Institute of Medical Physiology, University of Belgrade, Visegradska 26/II, RS-11129Belgrade, Serbia University of Haifa, 199 Abba Hushi Blvd., Mount Carmel, Haifa, IL-3498838, Israel
Rada Jeremic
Affiliation:
Faculty of Medicine, Institute of Medical Physiology, University of Belgrade, Visegradska 26/II, RS-11129Belgrade, Serbia
Sanja Dacic
Affiliation:
Department for General Physiology and Biophysics, Institute of Physiology and Biochemistry “Ivan Djaja”, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000Belgrade, Serbia
Sanja Pekovic
Affiliation:
Department of Neurobiology, Institute for Biological Research “Sinisa Stankovic”- National Institute of Republic of Serbia, University of Belgrade, Blvd despota Stefana 142, Belgrade, Serbia
Senka Pantic
Affiliation:
School of Medicine, Institute of Histology and Embryology, University of Belgrade, Visegradska 26/II, RS-11129Belgrade, Serbia
Marina Djelic
Affiliation:
Faculty of Medicine, Institute of Medical Physiology, University of Belgrade, Visegradska 26/II, RS-11129Belgrade, Serbia
Zagorka Vitic
Affiliation:
Department of Physiology and Cell Biology, Zlotowski Center for Neuroscience, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beersheba, Israel
Predrag Brkic
Affiliation:
Faculty of Medicine, Institute of Medical Physiology, University of Belgrade, Visegradska 26/II, RS-11129Belgrade, Serbia
Claude Brodski*
Affiliation:
Department of Physiology and Cell Biology, Zlotowski Center for Neuroscience, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beersheba, Israel
*
*Author for correspondence: Claude Brodski, E-mail: claude@bgu.ac.il
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Abstract

Traumatic brain injury (TBI) is a main cause of death and disabilities in young adults. Although learning and memory impairments are a major clinical manifestation of TBI, the consequences of TBI on the hippocampus are still not well understood. In particular, how lesions to the sensorimotor cortex damage the hippocampus, to which it is not directly connected, is still elusive. Here, we study the effects of sensorimotor cortex ablation (SCA) on the hippocampal dentate gyrus, by applying a highly sensitive gray-level co-occurrence matrix (GLCM) analysis. Using GLCM analysis of granule neurons, we discovered, in our TBI paradigm, subtle changes in granule cell (GC) morphology, including textual uniformity, contrast, and variance, which is not detected by conventional microscopy. We conclude that sensorimotor cortex trauma leads to specific changes in the hippocampus that advance our understanding of the cellular underpinnings of cognitive impairments in TBI. Moreover, we identified GLCM analysis as a highly sensitive method to detect subtle changes in the GC layers that is expected to significantly improve further studies investigating the impact of TBI on hippocampal neuropathology.

Keywords

dentate gyrusgray-level co-occurrence matrix analysishippocampussensorimotor cortex ablationtexturetraumatic brain injury
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 26 , Issue 1 , February 2020 , pp. 166 - 172
Copyright
Copyright © Microscopy Society of America 2020

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Footnotes

a

These authors contributed equally to this work.

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