Hostname: page-component-cd9895bd7-hc48f Total loading time: 0 Render date: 2024-12-28T17:30:55.675Z Has data issue: false hasContentIssue false
  • English
  • Français

Hemagglutinin-Catalyzed Cell-Cell Fusion: Kinetics of Initial Pore Formation from Video Rate, Multi-Wavelength Fluorescence Microscopy

Published online by Cambridge University Press:  08 August 2003

Stephen J. Morris ,
Daniel E. Howard ,
Teng H. Chang ,
Debi P. Sarkar  and
Robert Blumenthal
Stephen J. Morris
Affiliation:
Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO 64110-2499
Daniel E. Howard
Affiliation:
Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO 64110-2499
Teng H. Chang
Affiliation:
Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO 64110-2499
Debi P. Sarkar
Affiliation:
Department of Biochemistry, University of Delhi South Campus, New Delhi, India
Robert Blumenthal
Affiliation:
Section on Membrane Structure and Function, Laboratory of Mathematical Biology, National Cancer Institute, NIH, Bethesda, MD 20892
Get access

Abstract

Cell-cell fusion induced by the hemagglutinin (HA) spike-glycoprotein of influenza virus has been studied extensively using transfected cells expressing the protein. The initial fusion event is the formation of a small pore connecting the two cells. Its appearance has been measured by sudden changes in capacitance by whole cell patch clamp and more recently by the sudden change in the fluorescence of a possibly potential-sensitive fluorophore. Using a new design of fluorescence video microscopy capable of capturing multiple images at differing wavelengths of light simultaneously at video rates, this fluorescence event is shown to follow exponential kinetics, with a life time, τ, of approximately 350 ms.

Type
Research Article
Information
Microscopy and Microanalysis , Volume 1 , Issue 2 , June 1995 , pp. 48 - 54
Copyright
© 1995 Microscopy Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

This article is dedicated to Dr. Eric M. Shooter, Professor, Department of Neurobiology, Stanford University School of Medicine, on the occasion of his 70th birthday, April 18, 1994.