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High Resolution Immuno-Electron Microscopy Reveals That Fetal Skin Contains Microfibrils which are Heteropolymers of Fibrillin-1 and Fibrillin-2

Published online by Cambridge University Press:  02 July 2020

D.R. Keene ,
N.L. Charbonneau ,
B.J. Dzamba ,
D.P. Reinhardt ,
C.C. Ridgway ,
R.N. Ono  and
L.Y. Sakai
D.R. Keene
Affiliation:
Research Unit, Shriners Hospital for Children, Portland, Oregon97201
N.L. Charbonneau
Affiliation:
Research Unit, Shriners Hospital for Children, Portland, Oregon97201
B.J. Dzamba
Affiliation:
Research Unit, Shriners Hospital for Children, Portland, Oregon97201
D.P. Reinhardt
Affiliation:
Research Unit, Shriners Hospital for Children, Portland, Oregon97201
C.C. Ridgway
Affiliation:
Research Unit, Shriners Hospital for Children, Portland, Oregon97201
R.N. Ono
Affiliation:
Research Unit, Shriners Hospital for Children, Portland, Oregon97201
L.Y. Sakai
Affiliation:
Research Unit, Shriners Hospital for Children, Portland, Oregon97201
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Extract

Immunolocalization studies of neonate and older human tissues have previously demonstrated that fibrillin-1 is a component of elastin and non-elastin associated microfibrils. In sections taken from fixed, dehydrated and embedded tissue, microfibrils appear in the transmission electron microscope as hollow rods, 6-8 nm in diameter. When isolated from tissue by homogenization and observed following negative staining or rotary shadowing, microfibrils appear as beaded strings with a degree of extendibility. Recently, a closely related glycoprotein, fibrillin-2, has been described, which is expressed in early fetal development, prior to fibrillin-1, but then disappears in most tissues just prior to birth. We demonstrate here the characterization monoclonal antibodies specific for fibrillin-2. The antibodies are shown by ELISA and immunoblots to be fibrillin-2 specific. The matrix from a variety of tissues, including aorta, tendon, and eye are shown to contain fibrillin-2 by immunofluorescence in a pattern similar to that of fibrillin-1.

Type
Developmental Biology
Information
Microscopy and Microanalysis , Volume 4 , Issue S2: Proceedings: Microscopy & Microanalysis '98, Microscopy Society of America 56th Annual Meeting, Microbeam Analysis Society 32nd Annual Meeting, Atlanta, Georgia July 12-16, 1998 , July 1998 , pp. 1162 - 1163
Copyright
Copyright © Microscopy Society of America

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References

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