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High-Resolution Imaging of Kidney Vascular Corrosion Casts with Nano-CT

Published online by Cambridge University Press:  02 December 2010

Roger Wagner ,
Denis Van Loo ,
Fred Hossler ,
Kirk Czymmek ,
Elin Pauwels  and
Luc Van Hoorebeke
Roger Wagner*
Affiliation:
Department of Biological Sciences, University of Delaware, Newark, Delaware 19716, USA
Denis Van Loo
Affiliation:
UGCT, Department of Physics and Astronomy, Ghent University, Ghent, Belgium Department of Soil Management and Soil Care, Ghent University, Ghent, Belgium
Fred Hossler
Affiliation:
Department of Anatomy and Cell Biology, Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee, USA
Kirk Czymmek
Affiliation:
Department of Biological Sciences, University of Delaware, Newark, Delaware 19716, USA
Elin Pauwels
Affiliation:
UGCT, Department of Physics and Astronomy, Ghent University, Ghent, Belgium
Luc Van Hoorebeke
Affiliation:
UGCT, Department of Physics and Astronomy, Ghent University, Ghent, Belgium
*
Corresponding author. E-mail: Rags@udel.edu
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Abstract

A vascular corrosion cast of an entire mouse kidney was scanned with a modular multiresolution X-ray nanotomography system. Using an isotropic voxel pitch of 0.5 μm, capillary systems such as the vasa recta, peritubular capillaries and glomeruli were clearly resolved. This represents a considerable improvement over corrosion casts scanned with microcomputed tomography systems. The resolving power of this system was clearly demonstrated by the unique observation of a dense, subcapsular mat of capillaries enveloping the entire outer surface of the cortical region. Resolution of glomerular capillaries was comparable to similar models derived from laser scanning confocal microscopy. The high-resolution, large field of view and the three-dimensional nature of the resulting data opens new possibilities for the use of corrosion casting in research.

Keywords

nano-CTX-ray tomographycorrosion castscomputer modeling
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 17 , Issue 2 , April 2011 , pp. 215 - 219
Copyright
Copyright © Microscopy Society of America 2011

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