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High-Resolution Imaging of the Distributions of Cholesterol, Sphingolipids, and Specific Proteins in the Plasma Membrane with Secondary Ion Mass Spectrometry

Published online by Cambridge University Press:  23 September 2015

Mary L. Kraft ,
Peter K. Weber ,
Jessica F. Frisz ,
Haley A. Klitzing ,
Robert Wilson ,
Ashley Yeager  and
Joshua Zimmerberg
Mary L. Kraft
Affiliation:
School of Chemical Sciences, University of Illinois, 600 S. Mathews Avenue, Urbana, IL 61801, USA.
Peter K. Weber
Affiliation:
Glenn T. Seaborg Institute, Lawrence Livermore National Laboratory, 7000 East Ave., Livermore CA 94551, USA.
Jessica F. Frisz
Affiliation:
School of Chemical Sciences, University of Illinois, 600 S. Mathews Avenue, Urbana, IL 61801, USA.
Haley A. Klitzing
Affiliation:
School of Chemical Sciences, University of Illinois, 600 S. Mathews Avenue, Urbana, IL 61801, USA.
Robert Wilson
Affiliation:
School of Chemical Sciences, University of Illinois, 600 S. Mathews Avenue, Urbana, IL 61801, USA.
Ashley Yeager
Affiliation:
School of Chemical Sciences, University of Illinois, 600 S. Mathews Avenue, Urbana, IL 61801, USA.
Joshua Zimmerberg
Affiliation:
Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.

Abstract

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Type
Abstract
Information
Microscopy and Microanalysis , Volume 21 , Supplement S3: Proceedings of Microscopy & Microanalysis 2015 , August 2015 , pp. 2397 - 2398
Copyright
Copyright © Microscopy Society of America 2015 

References

[1] Klitzing., HA, et al., Secondary Ion Mass Spectrometry Imaging of Biological Membranes at High Spatial Resolution. In Methods in Molecular Biology: Nanoimaging Methods and Protocols, Sousa, A. A. & Kruhlak, M. J. (eds.) Humana Press, Totowa, New Jersey (2013) Vol. 950, pp 483.Google Scholar
[2] Frisz, JF, et al., Proc. Nat. Acad. Sci. U.S.A. (2013) 110(8), E613.Google Scholar
[3] Frisz, JF, et al., J. Biol. Chem. (2013) 288(23), 16855.Google Scholar
[4] The authors acknowledge funding from the Burroughs Wellcome Fund, the Intramural Program of the NICHD, NIH, the NIH Training Program in the Chemistry-Biology Interface T32 GM070421, the NSF under CHE-1058809, and Lab Directed Research and Development funding to LLNL. Dr. Kaiyan Lou is thanked for the synthesis of the 15N-sphingolipid precursors and 18O-cholesterol. Work at LLNL was supported by Lab Directed Research and Development funding and performed under the auspices of the U.S. DOE..Google Scholar