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High-Resolution Transmission Electron Microscopy Study of Nanostructured Hydroxyapatite

Published online by Cambridge University Press:  16 September 2008

Daniel Biggemann ,
Marcelo H. Prado da Silva ,
Alexandre M. Rossi  and
Antonio J. Ramirez
Daniel Biggemann
Affiliation:
Brazilian Synchrotron Light Laboratory, Caixa Postal 6192, Campinas-SP 13083-970, Brazil
Marcelo H. Prado da Silva
Affiliation:
Military Institute of Engineering, Pça. Gen. Tiburcio 80, Rio de Janeiro-RJ 22290-270, Brazil
Alexandre M. Rossi
Affiliation:
Brazilian Center for Physics Research, Rua Xavier Sigaud 150, Rio de Janeiro-RJ 22290-180, Brazil
Antonio J. Ramirez*
Affiliation:
Brazilian Synchrotron Light Laboratory, Caixa Postal 6192, Campinas-SP 13083-970, Brazil
*
Corresponding author. E-mail: ramirez@lnls.br
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Abstract

Crystalline properties of synthetic nanostructured hydroxyapatite (n-HA) were studied using high-resolution transmission electron microscopy. The focal-series-restoration technique, obtaining exit-plane wavefunction and spherical aberration-corrected images, was successfully applied for the first time in this electron-beam-susceptible material. Multislice simulations and energy dispersive X-ray spectroscopy were also employed to determine unequivocally that n-HA particles of different size preserve stoichiometric HA-like crystal structure. n-HA particles with sizes of twice the HA lattice parameter were found. These results can be used to optimize n-HA sinterization parameters to improve bioactivity.

Keywords

hydroxyapatiteHRTEMfocal seriesnanoparticles
Type
Materials Applications
Information
Microscopy and Microanalysis , Volume 14 , Issue 5 , October 2008 , pp. 433 - 438
Copyright
Copyright © Microscopy Society of America 2008

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References

REFERENCES

Kay, M.I., Young, R.A. & Posner, A.R. (1964). Crystal structure of hydroxyapatite. Nature 204, 1050–1052.CrossRefGoogle ScholarPubMed
Kumta, P.N., Sfeir, Ch., Lee, D.H., Olton, D. & Choi, D. (2005). Nanostructured calcium phosphates for biomedical applications: Novel synthesis and characterization. Acta Biomater 1, 65–83.CrossRefGoogle ScholarPubMed
Leventouri, Th., Bunaciu, C.E. & Perdikatsis, V. (2003). Neutron powder diffraction studies of silicon-substituted hydroxyapatite. Biomater 24, 4205–4211.CrossRefGoogle ScholarPubMed
Liu, J., Li, K., Wang, H., Zhu, M., Xu, H. & Yan, H. (2005). Self-assembly of hydroxyapatite nanostructures by microwave irradiation. Nanotechnology 16, 82–87.CrossRefGoogle Scholar
Mavropoulos, E., Rocha, N.C.C., Moreira, J.C., Rossi, A.M. & Soares, G.A. (2004). Characterization of phase evolution during lead immobilization by synthetic hydroxyapatite. Mater Charact 53, 71–78.CrossRefGoogle Scholar
Meyer, R.R., Kirkland, A.I. & Saxton, W.O. (2002). A new method for the determination of the wave aberration function for high resolution TEM 1. Measurement of the symmetric aberrations. Ultramicroscopy 92, 89–109.CrossRefGoogle ScholarPubMed
Rossi, A.M., Prado da Silva, M.H., Ramirez, A.J., Biggemann, D., Caraballo, M.M., Mascarenhas, Y.P., Eon, J.G. & Moure, G.T. (2007). Structural properties of hydroxyapatite with particle size less than 10 nanometers. Key Eng Mater 330, 255–258.CrossRefGoogle Scholar
Scherzer, O. (1948). The theoretical resolution limit of the electron microscope. J Appl Phys 20, 20–29.CrossRefGoogle Scholar
Stadelmann, P. (1987). EMS—A software package for electron diffraction analysis and HREM image simulation in materials science. Ultramicroscopy 21, 131–146.CrossRefGoogle Scholar
Suvorova, E.I. & Buffat, P.A. (1999). Electron diffraction from micro- and nanoparticles of hydroxyapatite. J Microsc 196, 46–58.CrossRefGoogle ScholarPubMed
Suvorova, E.I., Christensson, F., Lundager Madsen, H.E. & Chernov, A.A. (1998). Terrestrial and space-grown HAP and OCP crystals: Effect of growth conditions on perfection and morphology. J Cryst Growth 186, 262–274.CrossRefGoogle ScholarPubMed
Wang, Y.J., Lai, Ch., Wie, K., Chen, X., Ding, Y. & Wang, Z.L. (2006). Investigations on the formation mechanism of hydroxyapatite synthesized by the solvothermal method. Nanotechnology 17, 4405–4412.CrossRefGoogle Scholar
Zhu, X., Eibl, O., Berthold, Ch., Scheideler, L. & Geis-Gerstorfer, J. (2006). Structural characterization of nanocrystalline hydroxyapatite and adhesion of pre-osteoblast cells. Nanotechnology 17, 2711–2721.CrossRefGoogle Scholar