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A High-throughput Electron Microscopy Workflow and its Applications in Life Sciences

Published online by Cambridge University Press:  22 July 2022

Guido Ridolfi*
Affiliation:
Delmic, Fast Imaging, Delft, The Netherlands
Marre Niessen
Affiliation:
Delmic, Fast Imaging, Delft, The Netherlands
Melanie Adams Cioaba
Affiliation:
 Thermo Fisher Scientific, Hillsboro, Oregon, USA
Ruth Chalmers
Affiliation:
 Thermo Fisher Scientific, Hillsboro, Oregon, USA
Remco Jager
Affiliation:
Technolution, Gouda, The Netherlands
Gerard Rauwerda
Affiliation:
Technolution, Gouda, The Netherlands
*
*Corresponding author: ridolfi@delmic.com

Abstract

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Type
Artificial Intelligence, Instrument Automation, And High-dimensional Data Analytics for Microscopy and Microanalysis
Copyright
Copyright © Microscopy Society of America 2022

References

Kruit, P. and Zuidema, W., “A Dedicated Multi-Beam SEM for Transmission Imaging of Thin Samples,” Microsc. Microanal., vol. 25, no. S2, pp. 10341035, 2019.10.1017/S1431927619005907CrossRefGoogle Scholar
Ren, Y. and Kruit, P., “Transmission electron imaging in the Delft multibeam scanning electron microscope 1,J. Vac. Sci. Technol. B, Nanotechnol. Microelectron. Mater. Process. Meas. Phenom., vol. 34, no. 6, p. 06KF02, 2016.Google Scholar
Zuidema, W. and Kruit, P., “Transmission imaging on a scintillator in a scanning electron microscope,” Ultramicroscopy, vol. 218, p. 113055, 2020.Google Scholar
de Boer, P. et al. , “Large-scale electron microscopy database for human type 1 diabetes,” Nat. Commun., vol. 11, no. 1, pp. 19, 2020.10.1038/s41467-020-16287-5CrossRefGoogle ScholarPubMed
Templier, T. (2019). MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy. ELife, 8, 1. https://doi.org/10.7554/elife.45696CrossRefGoogle ScholarPubMed
European Regional Development Fund. Funded as part of the Union's response to the COVID-19 pandemic.Google Scholar