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Histomorphology of the Malpighian Tubules and the Chemical Composition of the Spherocrystals in the Tubule Epithelial Cells of Adult Leptophyes albovittata (Kollar, 1833) (Orthoptera, Tettigoniidae)

Published online by Cambridge University Press:  04 August 2021

Damla Amutkan Mutlu Open the ORCID record for Damla Amutkan Mutlu [Opens in a new window] ,
Irmak Polat Open the ORCID record for Irmak Polat [Opens in a new window]  and
Zekiye Suludere Open the ORCID record for Zekiye Suludere [Opens in a new window]
Damla Amutkan Mutlu
Affiliation:
Faculty of Science, Department of Biology, Gazi University, Ankara06500, Turkey
Irmak Polat*
Affiliation:
Faculty of Science, Department of Biology, Çankırı Karatekin University, Çankırı18100, Turkey
Zekiye Suludere
Affiliation:
Faculty of Science, Department of Biology, Gazi University, Ankara06500, Turkey
*
*Corresponding author: Irmak Polat, E-mail: irmakyilmaz@gazi.edu.tr
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Abstract

In insects, the number, cytological and histological structures, and the spherocrystals of the Malpighian tubules (MTs) can vary considerably in different insect groups. These differences are considered important because they can be used as taxonomic characters. For this purpose, the ultrastructure of the MT epithelial cells in Leptophyes albovittata (Kollar, 1833) (Orthoptera, Tettigoniidae) was examined by light microscopy, scanning electron microscopy, and transmission electron microscopy. The wall of each tubule consists of a single layer of cells. These cells have round-shaped nuclei. Two different cell types were demonstrated in the tubule cell. These are cells that have electron-dense cytoplasm and electron-lucent cytoplasm. It was observed that the cytoplasm of these cells has many spherocrystals. The chemical composition of the spherocrystals was found to be high in carbon, phosphorus, and manganese in tubule cells.

Keywords

electron microscopeelemental analysisexcretory systemlight microscopeSEM-EDX
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 27 , Issue 5 , October 2021 , pp. 1174 - 1182
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the Microscopy Society of America

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