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Imaging of Dental Hard Tissue Surfaces Prepared by an Ultrashort Pulsed Laser System (USPL)

Published online by Cambridge University Press:  12 December 2016

Matthias Schäper ,
Susanne Reimann ,
Matthias Frentzen  and
Jörg Meister
Matthias Schäper
Affiliation:
Department of Operative and Preventive Dentistry, Dental Faculty, Bonn University, 53111 Bonn, Germany
Susanne Reimann
Affiliation:
Oral Technology, Dental Faculty, Bonn University, 53111 Bonn, Germany
Matthias Frentzen
Affiliation:
Department of Operative and Preventive Dentistry, Dental Faculty, Bonn University, 53111 Bonn, Germany
Jörg Meister*
Affiliation:
Department of Operative and Preventive Dentistry, Dental Faculty, Bonn University, 53111 Bonn, Germany Department of Conservative Dentistry, Periodontology and Preventive Dentistry, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany
*
*Corresponding author. jmeister@uni-bonn.de
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Abstract

The aim of this study was to compare surface structures of laser-irradiated dental hard tissues using confocal (CFM), atomic force (AFM), and scanning electron microscopy (SEM). The general potential of the AFM in analyzing laser-irradiated surfaces was determined in this context. Specimens of human enamel and dentin were irradiated using an 8.6 W Nd:YVO4 laser with a pulse duration of 8 ps, λCenter=1,064 nm, and a pulse repetition rate of 500 kHz. Surface topology of irradiated areas (1 mm2) was investigated using AFM, CFM, and SEM. Surface roughness Rz was measured only with the AFM and the CFM. For non-irradiated enamel and dentin surfaces, roughnesses for CFM and AFM are in the nanometer range. However, major differences in roughness were determined for laser-prepared surfaces. For enamel, Rz(CFM)=2.33 μm is much higher compared with Rz(AFM)=0.09 μm; in the case of dentin, Rz(CFM)=5.35 μm is also much higher compared with Rz(AFM)=0.093 μm. Information regarding structural properties of surfaces needs real dimensions, particularly for use in dentistry. In this respect, AFM technology provides no additional results that lead to a significant improvement.

Keywords

imaging methodsultrashort pulsed laserenameldentinsurface topology
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 22 , Issue 6 , December 2016 , pp. 1189 - 1197
Copyright
© Microscopy Society of America 2016 

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