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Imaging of Vascular Smooth Muscle Cells with Soft X-Ray Spectromicroscopy

Published online by Cambridge University Press:  09 November 2011

Julia Sedlmair ,
Sophie-Charlotte Gleber ,
Semra Öztürk Mert ,
Michael Bertilson ,
Olov von Hofsten ,
Jürgen Thieme  and
Thomas Pfohl
Julia Sedlmair*
Affiliation:
Institute for X-Ray Physics, Georg-August-University Göttingen, Friedrich-Hund-Pl. 1, D-37077 Göttingen, Germany
Sophie-Charlotte Gleber
Affiliation:
Argonne National Laboratory, APS, 9700 S. Cass Avenue, Building 401, Argonne, IL 60439-4837, USA
Semra Öztürk Mert
Affiliation:
Max-Planck-Institute for Dynamics and Self-Organization, Bunsenstr. 10, D-37073, Göttingen, Germany
Michael Bertilson
Affiliation:
Biomedical and X-Ray Physics, Department of Applied Physics, Royal Institute of Technology, AlbaNova, SE-106 91 Stockholm, Sweden
Olov von Hofsten
Affiliation:
Biomedical and X-Ray Physics, Department of Applied Physics, Royal Institute of Technology, AlbaNova, SE-106 91 Stockholm, Sweden
Jürgen Thieme
Affiliation:
Brookhaven National Laboratory, NSLS II, Building 817, Upton, NY 11973, USA
Thomas Pfohl
Affiliation:
Max-Planck-Institute for Dynamics and Self-Organization, Bunsenstr. 10, D-37073, Göttingen, Germany Department of Chemistry, University of Basel, Klingelbergstr. 80, CH-4056, Basel, Switzerland
*
Corresponding author. E-mail: jsedlma@gwdg.de
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Abstract

Using X-ray microscopy and spectromicroscopy, vascular smooth muscle cells (VSMCs) were imaged, prepared without using additional embedding material or staining, but by applying simple, noncryo fixation techniques. The cells were imaged with a compact source transmission X-ray microscope and a scanning transmission X-ray microscope (STXM). With the STXM, spectromicroscopy was performed at the C K-edge and the Ca LIII,II-edges. VSMCs were chosen because of their high amount of actin stress fibers, so that the actin cytoskeleton should be visible. Other parts of the cell, such as the nucleus and organelles, were also identified from the micrographs. Both in the spectra and the images, the effects of the different preparation procedures were observable. Furthermore, Ca hotspots were detected and their density is determined.

Keywords

X-ray microscopyvascular smooth muscle cellsspectromicroscopyNEXAFScalciumradiation damage
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 17 , Issue 6 , December 2011 , pp. 991 - 1001
Copyright
Copyright © Microscopy Society of America 2011

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